Predictive and Carcinogenic Roles of Necroptosis-Related miR-425-5p and miR-16-5p in Esophageal Squamous Cell Carcinoma.

IF 1.1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY Annals of clinical and laboratory science Pub Date : 2024-01-01

Xue Zhidan, Xi Kedi, Xi Dan, Song Xing, Sun Rui

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引用次数: 0

Abstract

Objective: To examine the expression and function of necroptosis-associated miRNAs in esophageal squamous cell carcinoma.

Methods: A total of three microarray datasets, i.e., GSE122497, GSE114110, and GSE43732, were selected from the GEO database for differential analysis of necroptosis-related miRNA expression. The differentially expressed miRNAs were screened for target miRNAs using Kaplan-Meier survival analysis in the OncomiR database. The expression of the target miRNAs in the HEEC, KYSE-450, TE-1, and KYSE-410 cell lines was measured via qPCR. The expression of the target miRNAs in esophageal cancer cells was regulated by transfection with Lipofectamine 2000, and cell proliferation, cell migration, cell apoptosis and the cell cycle were detected by CCK-8, Transwell, and flow cytometry.

Results: The tumor tissue and peripheral blood of esophageal squamous cell cancer patients showed differential expression of 7 miRNAs related to necroptosis. Survival analysis revealed that miR-425-5p and miR-16-5p were negatively correlated with patient survival. The esophageal squamous cell carcinoma cell lines exhibited increased expression of miR-425-5p and miR-16-5p, with KYSE-410 exhibiting the most significant increase. Inhibition of miR-425-5p and miR-16-5p expression in the KYSE-410 cell line resulted in increased apoptosis, decreased proliferation, and decreased migration of esophageal cancer cells as well as a significant increase in the S phase and a decrease in the G2/M phase according to the cell cycle assay.

Conclusion: The pro-carcinogenic role of miR-425-5p and miR-16-5p has been observed in esophageal squamous cell carcinoma.

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与坏死相关的 miR-425-5p 和 miR-16-5p 在食管鳞状细胞癌中的预测作用和致癌作用

目的：研究食管鳞状细胞癌坏死相关 miRNA 的表达和功能：研究食管鳞状细胞癌坏死相关miRNA的表达和功能：从 GEO 数据库中选取 GSE122497、GSE114110 和 GSE43732 这三个芯片数据集，对坏死相关 miRNA 的表达进行差异分析。利用 OncomiR 数据库中的 Kaplan-Meier 生存分析，筛选出差异表达的 miRNAs 作为目标 miRNA。通过 qPCR 测定目标 miRNA 在 HEEC、KYSE-450、TE-1 和 KYSE-410 细胞系中的表达。用 Lipofectamine 2000 转染食管癌细胞，并用 CCK-8、Transwell 和流式细胞术检测细胞增殖、细胞迁移、细胞凋亡和细胞周期：结果表明：食管鳞癌患者的肿瘤组织和外周血中与坏死相关的7种miRNA的表达存在差异。生存率分析表明，miR-425-5p 和 miR-16-5p 与患者生存率呈负相关。食管鳞状细胞癌细胞系的 miR-425-5p 和 miR-16-5p 表达增加，其中 KYSE-410 的增加最为显著。抑制 miR-425-5p 和 miR-16-5p 在 KYSE-410 细胞系中的表达会导致食管癌细胞凋亡增加、增殖减少、迁移降低，并且根据细胞周期测定，S 期显著增加，G2/M 期减少：结论：在食管鳞状细胞癌中观察到了 miR-425-5p 和 miR-16-5p 的促癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.