Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort

IF 4.2 2区医学 Q1 ENDOCRINOLOGY & METABOLISM Obesity Pub Date : 2024-03-22 DOI:10.1002/oby.24009

Bruna Rubbo, Zhenjiang Li, Phum Tachachartvanich, Brittney O. Baumert, Hongxu Wang, Shudi Pan, Sarah Rock, Justin R. Ryder, Todd Jenkins, Stephanie Sisley, Xiangping Lin, Scott Bartell, Thomas H. Inge, Stavra Xanthakos, Brooklynn McNeil, Anna R. Robuck, Michele A. La Merrill, Douglas I. Walker, David V. Conti, Rob McConnell, Sandrah P. Eckel, Lida Chatzi

{"title":"Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort","authors":"Bruna Rubbo, Zhenjiang Li, Phum Tachachartvanich, Brittney O. Baumert, Hongxu Wang, Shudi Pan, Sarah Rock, Justin R. Ryder, Todd Jenkins, Stephanie Sisley, Xiangping Lin, Scott Bartell, Thomas H. Inge, Stavra Xanthakos, Brooklynn McNeil, Anna R. Robuck, Michele A. La Merrill, Douglas I. Walker, David V. Conti, Rob McConnell, Sandrah P. Eckel, Lida Chatzi","doi":"10.1002/oby.24009","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>Dichlorodiphenyldichloroethylene (DDE), an obesogen accumulating in adipose tissue, is released into circulation with weight loss, although its impact is underexplored among adolescents. We tested the association using an integrative translational approach of epidemiological analysis among adolescents with obesity and <i>in vitro</i> measures exploring the impact of DDE on adipogenesis via preadipocytes.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>We included 63 participants from the Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS) cohort. We assessed 4,4′-DDE in visceral adipose tissue at surgery and BMI and waist circumference at surgery and 0.5, 1, 3, and 5 years after. We conducted longitudinal analysis to estimate the interaction on weight loss between DDE and time since surgery. <i>In vitro</i> analysis quantified adipogenic differentiation in commercial human preadipocytes exposed to 4,4′-DDE via fluorescent staining and imaging.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>A dose–response relationship was observed, with the low-exposure group having a greater reduction in BMI during the first year compared to higher-exposure groups and showing smaller regains compared to higher-exposure groups after the first year. <i>In vitro</i> analysis of preadipocytes treated with 4,4′-DDE during adipogenic differentiation for 12 days showed a concentration-dependent increase in lipid accumulation.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>DDE could contribute to weight trajectory among adolescents undergoing bariatric surgery, potentially mediated via promoted adipogenesis in preadipocytes.</p>\n </section>\n </div>","PeriodicalId":215,"journal":{"name":"Obesity","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/oby.24009","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Obesity","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/oby.24009","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Dichlorodiphenyldichloroethylene (DDE), an obesogen accumulating in adipose tissue, is released into circulation with weight loss, although its impact is underexplored among adolescents. We tested the association using an integrative translational approach of epidemiological analysis among adolescents with obesity and in vitro measures exploring the impact of DDE on adipogenesis via preadipocytes.

Methods

We included 63 participants from the Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS) cohort. We assessed 4,4′-DDE in visceral adipose tissue at surgery and BMI and waist circumference at surgery and 0.5, 1, 3, and 5 years after. We conducted longitudinal analysis to estimate the interaction on weight loss between DDE and time since surgery. In vitro analysis quantified adipogenic differentiation in commercial human preadipocytes exposed to 4,4′-DDE via fluorescent staining and imaging.

Results

A dose–response relationship was observed, with the low-exposure group having a greater reduction in BMI during the first year compared to higher-exposure groups and showing smaller regains compared to higher-exposure groups after the first year. In vitro analysis of preadipocytes treated with 4,4′-DDE during adipogenic differentiation for 12 days showed a concentration-dependent increase in lipid accumulation.

Conclusions

DDE could contribute to weight trajectory among adolescents undergoing bariatric surgery, potentially mediated via promoted adipogenesis in preadipocytes.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Teen-LABS 队列中青少年内脏脂肪组织中的 4,4'-DDE暴露与体重下降。

目的：二氯二苯基二氯乙烯（DDE）是一种积聚在脂肪组织中的肥胖原，会随着体重的减轻释放到血液循环中，但其对青少年的影响尚未得到充分研究。我们通过对肥胖青少年进行流行病学分析，并采用体外测量方法探索 DDE 通过前脂肪细胞对脂肪生成的影响，采用综合转化方法检验了两者之间的关联：我们从青少年减肥手术纵向评估（Teen-LABS）队列中纳入了 63 名参与者。我们评估了手术时内脏脂肪组织中的 4,4'-DDE，以及手术后 0.5 年、1 年、3 年和 5 年的体重指数和腰围。我们进行了纵向分析，以估计 DDE 与手术后时间对体重减轻的交互作用。体外分析通过荧光染色和成像对暴露于4,4'-DDE的商业化人类前脂肪细胞的成脂分化进行了量化：结果：观察到一种剂量-反应关系，与暴露程度较高的组相比，暴露程度较低的组在第一年中的体重指数下降幅度较大，与暴露程度较高的组相比，暴露程度较高的组在第一年后的体重指数恢复幅度较小。在体外分析中，用4,4'-DDE处理的前脂肪细胞在成脂分化过程中持续12天，结果显示脂质积累的增加与浓度有关：结论：DDE 可能通过促进前脂肪细胞的脂肪生成，对接受减肥手术的青少年的体重轨迹产生影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Obesity 医学-内分泌学与代谢

CiteScore

11.70

自引率

1.40%

发文量

261

审稿时长

2-4 weeks

期刊介绍： Obesity is the official journal of The Obesity Society and is the premier source of information for increasing knowledge, fostering translational research from basic to population science, and promoting better treatment for people with obesity. Obesity publishes important peer-reviewed research and cutting-edge reviews, commentaries, and public health and medical developments.