Isolation and Functional Analysis of Na+/H+ Antiporter Gene (LcNHX1) from Leymus chinensis

IF 1.4 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS Plant Molecular Biology Reporter Pub Date : 2024-03-22 DOI:10.1007/s11105-024-01446-5

Chuanbo Sun, Chuang Zhang, Yuejia Yin, Ying Wang, Shujing Mu, Qing Liu, Ziyu Wang, Yang Liu, Chunxiao Zhang, Xiangguo Liu, Yan Zhang, Jia Guo

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Abstract

Saline-alkali stress has adverse effects on plant growth. Some plant Na⁺/H⁺ antiporters were reported to be important in salt tolerance. However, it needs to be better understood that Na⁺/H⁺ antiporters are involved in plant salt-alkali (NaHCO₃/Na₂CO₃) tolerance. In this study, a Na⁺/H⁺ antiporter gene LcNHX1 (China patent No.200810050629.1) has been cloned from Leymus chinensis. The LcNHX1 CDS contains 1614 bp that encodes 537 amino acids. Amino acid and nucleotide sequence similarity, protein topology modelling, conserved functional domains in the protein sequence, and subcellular localization classified LcNHX1 as a vacuolar NHX1 homolog. Transcription analysis by quantitative RT-PCR indicated that upregulated expression of LcNHX1 could be induced by NaCl, NaHCO₃, NaCl + NaHCO₃, and PGE in L. chinensis seedlings. The expression of LcNHX1 partially complements the salt-sensitive phenotypes of a Δnhx1 yeast strain. In addition, LcNHX1 overexpressing enhanced the tolerance to NaHCO₃ stress in the transgenic Arabidopsis. Taken together, these results indicated that LcNHX1 is a potential candidate gene for enhancing plant saline-alkali tolerance.

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莱姆斯 chinensis 的 Na+/H+ 反转运体基因（LcNHX1）的分离与功能分析

盐碱胁迫对植物生长有不利影响。据报道，一些植物的 Na+/H+ 反转运体对耐盐性很重要。然而，Na+/H+拮抗剂是否参与植物耐盐碱（NaHCO3/Na2CO3）还需要进一步了解。本研究从Leymus chinensis中克隆了一个Na+/H+反转运体基因LcNHX1（中国专利号：200810050629.1）。LcNHX1 CDS包含1614 bp，编码537个氨基酸。氨基酸和核苷酸序列相似性、蛋白质拓扑模型、蛋白质序列中的保守功能域以及亚细胞定位将 LcNHX1 定义为空泡 NHX1 同源物。定量 RT-PCR 转录分析表明，NaCl、NaHCO3、NaCl + NaHCO3 和 PGE 均可诱导 LcNHX1 的表达。LcNHX1的表达部分补充了Δnhx1酵母菌株的盐敏感表型。此外，LcNHX1的过表达增强了转基因拟南芥对NaHCO3胁迫的耐受性。综上所述，这些结果表明 LcNHX1 是增强植物耐盐碱能力的潜在候选基因。

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来源期刊

Plant Molecular Biology Reporter 生物-生化研究方法

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

2.7 months

期刊介绍： The scope of the journal of Plant Molecular Biology Reporter has expanded to keep pace with new developments in molecular biology and the broad area of genomics. The journal now solicits papers covering myriad breakthrough technologies and discoveries in molecular biology, genomics, proteomics, metabolomics, and other ‘omics’, as well as bioinformatics.