Physiological and transcriptome analysis provided insights for the response of yellowhorn to drought stress

IF 2.1 3区农林科学 Q2 FORESTRY Trees Pub Date : 2024-03-23 DOI:10.1007/s00468-024-02511-y

Yingying Yang, Xiaojuan Liu, Huihui Xu, Zhuo Ban, Ziquan Zhao, Quanxin Bi, Libing Wang

{"title":"Physiological and transcriptome analysis provided insights for the response of yellowhorn to drought stress","authors":"Yingying Yang, Xiaojuan Liu, Huihui Xu, Zhuo Ban, Ziquan Zhao, Quanxin Bi, Libing Wang","doi":"10.1007/s00468-024-02511-y","DOIUrl":null,"url":null,"abstract":"<div><h3>Key message</h3><p>This study not only provide a theoretical basis for screening drought-resistant yellowhorn rootstocks but also lay the foundation for the research on the drought resistance mechanism of yellowhorn.</p><h3>Abstract</h3><p>Drought is a major limiting factor in the production and cultivation of yellowhorn, an important woody oil species in China. It is important to select excellent drought-resistant rootstocks, and the current reproduction of rootstocks of yellowhorn is mainly through seed propagation. In this study, we analyzed the phenotypes, physiology and anatomy of the F1 generation of five yellowhorn varieties (‘Zhongshi 1’, ‘Zhongshi 4’, ‘Zhongshi 7’, ‘Zhongshi 9’ and ‘Yuanda’) which are currently the main promoted varieties of yellowhorn. The changes in physiological traits were used to comprehensively rank drought resistance by the membership function method. The results showed that the drought resistance of different varieties was in the order ‘Zhongshi 9’ > ‘Zhongshi 4’ > ‘Yuanda’ > ‘Zhongshi 7’ > ‘Zhongshi 1’. In addition, we investigated the molecular signature of two yellowhorn varieties exhibiting a large difference in levels of drought resistance using transcriptome data. A total of 119 differentially expressed genes (DEGs) were found between the 2 yellowhorn varieties under drought stress. Based on KEGG analysis, DEGs were mostly enriched in three pathways. According to the correlation analysis between weighted gene co-expression network analysis (WGCNA) and physiological traits, a total of four co-expression networks with high correlation were constructed, and some hub genes of yellowhorn in response to drought stress were found. We carried out a comprehensive evaluation of yellowhorn drought tolerance based on parental lines. It solves the current problems faced in the breeding of yellowhorn and lays the foundation for studying the molecular mechanism of drought resistance in yellowhorn.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"38 3","pages":"725 - 742"},"PeriodicalIF":2.1000,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trees","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1007/s00468-024-02511-y","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FORESTRY","Score":null,"Total":0}

引用次数: 0

Key message

This study not only provide a theoretical basis for screening drought-resistant yellowhorn rootstocks but also lay the foundation for the research on the drought resistance mechanism of yellowhorn.

Abstract

Drought is a major limiting factor in the production and cultivation of yellowhorn, an important woody oil species in China. It is important to select excellent drought-resistant rootstocks, and the current reproduction of rootstocks of yellowhorn is mainly through seed propagation. In this study, we analyzed the phenotypes, physiology and anatomy of the F1 generation of five yellowhorn varieties (‘Zhongshi 1’, ‘Zhongshi 4’, ‘Zhongshi 7’, ‘Zhongshi 9’ and ‘Yuanda’) which are currently the main promoted varieties of yellowhorn. The changes in physiological traits were used to comprehensively rank drought resistance by the membership function method. The results showed that the drought resistance of different varieties was in the order ‘Zhongshi 9’ > ‘Zhongshi 4’ > ‘Yuanda’ > ‘Zhongshi 7’ > ‘Zhongshi 1’. In addition, we investigated the molecular signature of two yellowhorn varieties exhibiting a large difference in levels of drought resistance using transcriptome data. A total of 119 differentially expressed genes (DEGs) were found between the 2 yellowhorn varieties under drought stress. Based on KEGG analysis, DEGs were mostly enriched in three pathways. According to the correlation analysis between weighted gene co-expression network analysis (WGCNA) and physiological traits, a total of four co-expression networks with high correlation were constructed, and some hub genes of yellowhorn in response to drought stress were found. We carried out a comprehensive evaluation of yellowhorn drought tolerance based on parental lines. It solves the current problems faced in the breeding of yellowhorn and lays the foundation for studying the molecular mechanism of drought resistance in yellowhorn.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

生理和转录组分析为黄角树应对干旱胁迫提供了启示

摘要干旱是我国重要木本油料树种黄角树生产和栽培的主要限制因素。选择优良的抗旱砧木非常重要，目前黄角砧木的繁殖主要通过种子繁殖。本研究分析了目前主要推广的五个黄角品种（'中石1号'、'中石4号'、'中石7号'、'中石9号'和'远大'）F1代的表型、生理和解剖特征。利用生理性状的变化，采用成员函数法对抗旱性进行综合排名。结果表明，不同品种的抗旱性依次为'中植 9 号'> '中植 4 号'> '远大'> '中植 7 号'> '中植 1 号'。此外，我们还利用转录组数据研究了抗旱性差异较大的两个黄角品种的分子特征。结果发现，两个黄角品种在干旱胁迫下共有 119 个差异表达基因（DEGs）。根据 KEGG 分析，DEGs 主要富集在三个通路中。根据加权基因共表达网络分析（WGCNA）与生理性状的相关性分析，共构建了4个相关性较高的共表达网络，并发现了一些黄角应对干旱胁迫的枢纽基因。我们基于亲本品系对黄角耐旱性进行了综合评价。它解决了目前黄角育种面临的问题，为研究黄角抗旱的分子机理奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Trees 农林科学-林学

CiteScore

4.50

自引率

4.30%

发文量

113

审稿时长

3.8 months

期刊介绍： Trees - Structure and Function publishes original articles on the physiology, biochemistry, functional anatomy, structure and ecology of trees and other woody plants. Also presented are articles concerned with pathology and technological problems, when they contribute to the basic understanding of structure and function of trees. In addition to original articles and short communications, the journal publishes reviews on selected topics concerning the structure and function of trees.