Exploring the therapeutic potential of pomegranate juice for uterine relaxation.

IF 2.7 Q3 ENDOCRINOLOGY & METABOLISM Expert Review of Endocrinology & Metabolism Pub Date : 2024-07-01 Epub Date: 2024-03-25 DOI:10.1080/17446651.2024.2334218

Omar A M Al-Habib, Lina N Adam

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Abstract

Background: The effects of pomegranate juice (PJ) and its components on uterine smooth muscle are unknown. Hence, this study unequivocally demonstrates that pomegranate juice (PJ) significantly impacts myometrial function, providing crucial insights into its relaxant properties and their potential therapeutic applications for uterine-related disorders.

Research design and methods: Rat uterine smooth muscle horn strips were suspended in Krebs solution organ baths. Contractions were measured isometrically using a transducer (AD instrument Australia). The effects of PJ were evaluated on contractile activity elicited by potassium chloride (KCl 60 Mm) depolarization. Inhibitors of nitric oxide (L-NAME 3 X 10^-4), guanylate cyclase (methylene blue 1 X 10^-5), and Prostaglandin I₂ (indomethacin 3 X 10^-5), as well as Potassium Channels blockers, were determined.

Results: The juice at concentrations from 1.5-5 mg/ml significantly decreased the rat uterine horn contraction induced by KCl. The NO, cGMP, and PGI₂ inhibitors did not block the relaxation response. Furthermore, the PGI₂ inhibitor significantly enhanced the relaxation effects; K⁺ channel blockers had no inhibitory effects on the relaxation responses. Contrarily, GLIB improved considerably relaxation.

Conclusion: Research suggests pomegranate juice's active ingredient may reduce uterine contractions and treat uterotonic disorders, potentially preventing preterm birth and dysmenorrhea. Further research is needed to determine its mechanism of action.

Trial registration: Code: AEC-013.

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探索石榴汁对子宫松弛的治疗潜力。

背景：石榴汁及其成分对子宫平滑肌的影响尚不清楚。因此，本研究明确证明石榴汁（PJ）对子宫肌功能有显著影响，为了解其松弛特性及其对子宫相关疾病的潜在治疗应用提供了重要依据：将大鼠子宫平滑肌角带悬浮在克雷布斯溶液器官浴中。使用传感器（澳大利亚 AD 仪器）对收缩进行等距测量。评估了 PJ 对氯化钾（KCl 60 毫微米）去极化引起的收缩活动的影响。对一氧化氮抑制剂（L-NAME 3 X 10-4）、鸟苷酸环化酶抑制剂（亚甲基蓝 1 X 10-5）和前列腺素 I2 抑制剂（吲哚美辛 3 X 10-5）以及钾通道阻滞剂进行了测定：结果：浓度为 1.5-5 毫克/毫升的果汁能明显降低氯化钾引起的大鼠子宫角收缩。NO、cGMP 和 PGI2 抑制剂并未阻止松弛反应。此外，PGI2 抑制剂能明显增强松弛效应；K+ 通道阻滞剂对松弛反应没有抑制作用。与此相反，GLIB 则明显改善了松弛反应：研究表明，石榴汁的有效成分可减少子宫收缩，治疗子宫张力障碍，从而预防早产和痛经。需要进一步研究以确定其作用机制：试验注册：代码：AEC-013：试验注册：代码：AEC-013。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Endocrinology & Metabolism ENDOCRINOLOGY & METABOLISM-

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4.80

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0.00%

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期刊介绍： Implicated in a plethora of regulatory dysfunctions involving growth and development, metabolism, electrolyte balances and reproduction, endocrine disruption is one of the highest priority research topics in the world. As a result, we are now in a position to better detect, characterize and overcome the damage mediated by adverse interaction with the endocrine system. Expert Review of Endocrinology and Metabolism (ISSN 1744-6651), provides extensive coverage of state-of-the-art research and clinical advancements in the field of endocrine control and metabolism, with a focus on screening, prevention, diagnostics, existing and novel therapeutics, as well as related molecular genetics, pathophysiology and epidemiology.