Methylomonadaceae was the active and dominant methanotroph in Tibet lake sediments.

IF 5.1 Q1 ECOLOGY ISME communications Pub Date : 2024-03-04 eCollection Date: 2024-01-01 DOI:10.1093/ismeco/ycae032
Yongcui Deng, Chulin Liang, Xiaomeng Zhu, Xinshu Zhu, Lei Chen, Hongan Pan, Fan Xun, Ye Tao, Peng Xing
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Abstract

Methane (CH4), an important greenhouse gas, significantly impacts the local and global climate. Our study focused on the composition and activity of methanotrophs residing in the lakes on the Tibetan Plateau, a hotspot for climate change research. Based on the field survey, the family Methylomonadaceae had a much higher relative abundance in freshwater lakes than in brackish and saline lakes, accounting for ~92% of total aerobic methanotrophs. Using the microcosm sediment incubation with 13CH4 followed by high throughput sequencing and metagenomic analysis, we further demonstrated that the family Methylomonadaceae was actively oxidizing CH4. Moreover, various methylotrophs, such as the genera Methylotenera and Methylophilus, were detected in the 13C-labeled DNAs, which suggested their participation in CH4-carbon sequential assimilation. The presence of CH4 metabolism, such as the tetrahydromethanopterin and the ribulose monophosphate pathways, was identified in the metagenome-assembled genomes of the family Methylomonadaceae. Furthermore, they had the potential to adapt to oxygen-deficient conditions and utilize multiple electron acceptors, such as metal oxides (Fe3+), nitrate, and nitrite, for survival in the Tibet lakes. Our findings highlighted the predominance of Methylomonadaceae and the associated microbes as active CH4 consumers, potentially regulating the CH4 emissions in the Tibet freshwater lakes. These insights contributed to understanding the plateau carbon cycle and emphasized the significance of methanotrophs in mitigating climate change.

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甲单胞菌是西藏湖泊沉积物中活跃的优势甲烷营养体。
甲烷(CH4)是一种重要的温室气体,对当地和全球气候有重大影响。我们的研究重点是青藏高原湖泊中甲烷营养体的组成和活性,青藏高原是气候变化研究的热点地区。根据实地调查,淡水湖泊中甲单胞菌科的相对丰度远高于咸水湖和盐湖,占需氧甲烷营养体总数的约92%。通过用 13CH4 培养微宇宙沉积物,然后进行高通量测序和元基因组分析,我们进一步证明了甲基单胞菌科能积极氧化 CH4。此外,我们还在 13C 标记的 DNA 中检测到了多种养甲菌,如甲单胞菌属(Methylotenera)和嗜甲单胞菌属(Methylophilus),这表明它们参与了 CH4 碳顺序同化。在甲基单胞菌科的元基因组组装基因组中,发现了四氢甲蝶呤和核酮糖单磷酸途径等CH4代谢的存在。此外,它们有可能适应缺氧条件,并利用多种电子受体(如金属氧化物(Fe3+)、硝酸盐和亚硝酸盐)在西藏湖泊中生存。我们的研究结果突显了甲基单胞菌科及其相关微生物作为活跃的CH4消费者的优势,它们有可能调节西藏淡水湖泊的CH4排放。这些见解有助于理解高原碳循环,并强调了甲烷营养体在减缓气候变化中的重要作用。
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