Properties of Carbonated Hydroxyapatite-Based Scaffold from Oyster Shells Composited with Honeycomb and Polyethylene Oxide for Bone Tissue Engineering Applications
{"title":"Properties of Carbonated Hydroxyapatite-Based Scaffold from Oyster Shells Composited with Honeycomb and Polyethylene Oxide for Bone Tissue Engineering Applications","authors":"Nilam Cahyati, M. Sari, Yusril Yusuf","doi":"10.4028/p-mh0ptj","DOIUrl":null,"url":null,"abstract":"Scaffold Carbonated Hydroxyapatite/Honeycomb/Polyethylene Oxide (CHA/HCB/PEO) has been obtained by freeze-drying. The bioceramic CHA used in this study was synthesized from oyster shells using precipitation. HCB and PEO were added as reinforcement materials that affect the crystallographic properties of the scaffold. This study aimed to determine the characteristics of the scaffolds for bone tissue engineering. CHA and scaffolds were characterized using Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometer (XRD), and Scanning Electron Microscopy (SEM). FTIR spectra and XRD graphs confirmed that the CHA produced was B-type. FTIR spectra of the scaffold showed the presence of HCB and PEO in the scaffold, which means they were homogeneously bound in the scaffold solution. XRD test results show that scaffolds' crystallinity and crystallite size tends to decrease compared to CHA. This was good because they could make cells easier to proliferate. A small-scale pore structure (micropore) was also formed in the scaffold. The porosity and pore size of the scaffold were affected by the concentration of CHA. The presence of the micropores can increase the permeability of the scaffold and facilitate cell migration. Thus, the composition of CHA/HCB/PEO scaffolds can be a good candidate material in bone tissue engineering.","PeriodicalId":17714,"journal":{"name":"Key Engineering Materials","volume":" 31","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Key Engineering Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4028/p-mh0ptj","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Scaffold Carbonated Hydroxyapatite/Honeycomb/Polyethylene Oxide (CHA/HCB/PEO) has been obtained by freeze-drying. The bioceramic CHA used in this study was synthesized from oyster shells using precipitation. HCB and PEO were added as reinforcement materials that affect the crystallographic properties of the scaffold. This study aimed to determine the characteristics of the scaffolds for bone tissue engineering. CHA and scaffolds were characterized using Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometer (XRD), and Scanning Electron Microscopy (SEM). FTIR spectra and XRD graphs confirmed that the CHA produced was B-type. FTIR spectra of the scaffold showed the presence of HCB and PEO in the scaffold, which means they were homogeneously bound in the scaffold solution. XRD test results show that scaffolds' crystallinity and crystallite size tends to decrease compared to CHA. This was good because they could make cells easier to proliferate. A small-scale pore structure (micropore) was also formed in the scaffold. The porosity and pore size of the scaffold were affected by the concentration of CHA. The presence of the micropores can increase the permeability of the scaffold and facilitate cell migration. Thus, the composition of CHA/HCB/PEO scaffolds can be a good candidate material in bone tissue engineering.
碳化羟基磷灰石/蜂窝/聚乙烯氧化物(CHA/HCB/PEO)支架是通过冷冻干燥法获得的。本研究中使用的生物陶瓷 CHA 是利用沉淀法从牡蛎壳中合成的。添加的 HCB 和 PEO 作为增强材料会影响支架的晶体学特性。本研究旨在确定骨组织工程支架的特性。使用傅立叶变换红外光谱仪(FTIR)、X 射线衍射仪(XRD)和扫描电子显微镜(SEM)对 CHA 和支架进行了表征。傅立叶变换红外光谱和 X 射线衍射图证实所生产的 CHA 为 B 型。支架的傅立叶变换红外光谱显示支架中存在六氯苯和聚乙烯醇,这意味着它们在支架溶液中结合均匀。XRD 测试结果表明,与 CHA 相比,支架的结晶度和晶粒尺寸呈下降趋势。这很好,因为它们能使细胞更容易增殖。支架中还形成了小规模的孔隙结构(微孔)。支架的孔隙率和孔径受 CHA 浓度的影响。微孔的存在可增加支架的渗透性,促进细胞迁移。因此,CHA/HCB/PEO 组成的支架可作为骨组织工程中的良好候选材料。