Revealing the specific regulations of nitric oxide on the postharvest ripening and senescence of bitter melon fruit

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY aBIOTECH Pub Date : 2024-03-21 DOI:10.1007/s42994-023-00110-y
Hongwei Wang, Ling Li, Lili Ma, Alisdair R. Fernie, Anzhen Fu, Chunmei Bai, Zhaoze Sang, Susu Guo, Fan Zhang, Qing Wang, Yanyan Zheng, Jinhua Zuo
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Abstract

Bitter melon fruit is susceptible to yellowing, softening, and rotting under room-temperature storage conditions, resulting in reduced commercial value. Nitric oxide (NO) is an important signaling molecule and plays a crucial role in regulating the fruit postharvest quality. In this study, we investigated the effects of NO treatment on changes in sensory and firmness of bitter melon fruit during postharvest storage. Moreover, transcriptomic, metabolomic, and proteomic analyses were performed to elucidate the regulatory mechanisms through which NO treatment delays the ripening and senescence of bitter melon fruit. Our results show that differentially expressed genes (DEGs) were involved in fruit texture (CSLE, β-Gal, and PME), plant hormone signal transduction (ACS, JAR4, and AUX28), and fruit flavor and aroma (SUS2, LOX, and GDH2). In addition, proteins differentially abundant were associated with fruit texture (PLY, PME, and PGA) and plant hormone signal transduction (PBL15, JAR1, and PYL9). Moreover, NO significantly increased the abundance of key enzymes involved in the phenylpropanoid biosynthetic pathway, thus enhancing the disease resistance and alleviating softening of bitter melon fruit. Finally, differential metabolites mainly included phenolic acids, terpenoids, and flavonoids. These results provide a theoretical basis for further studies on the physiological changes associated with postharvest ripening and senescence of bitter melon fruit.

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揭示一氧化氮对苦瓜果实采后成熟和衰老的特殊调节作用
在室温贮藏条件下,苦瓜果实容易变黄、软化和腐烂,导致商业价值降低。一氧化氮(NO)是一种重要的信号分子,在调节果实采后品质方面起着至关重要的作用。本研究调查了一氧化氮处理对采后贮藏期间苦瓜果实感官和硬度变化的影响。此外,我们还进行了转录组、代谢组和蛋白质组分析,以阐明氮氧化物处理延缓苦瓜果实成熟和衰老的调控机制。结果表明,差异表达基因(DEGs)涉及果实质地(CSLE、β-Gal和PME)、植物激素信号转导(ACS、JAR4和AUX28)以及果实风味和香气(SUS2、LOX和GDH2)。此外,与果实质地(PLY、PME 和 PGA)和植物激素信号转导(PBL15、JAR1 和 PYL9)相关的蛋白质丰度存在差异。此外,氮氧化物明显增加了参与苯丙类生物合成途径的关键酶的丰度,从而提高了苦瓜的抗病性,减轻了苦瓜果实的软化。最后,差异代谢产物主要包括酚酸类、萜类和黄酮类化合物。这些结果为进一步研究与苦瓜果实采后成熟和衰老相关的生理变化提供了理论依据。
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CiteScore
7.70
自引率
2.80%
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0
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