Modulating the Wnt Signaling Pathway in Wound Healing: The Potential of L-Carnosine, Zinc Sulfate, and B-Complex Vitamins Cocktail

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-03-13 DOI:10.5812/jjnpp-143129

Nafiseh Erfanian, Mahsa Saffar, Sahba Khatami, Emad Yeganeh Khorasani, Saboura Nikpour, Mohammad Fereidouni, M. Namaei

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Abstract

Background: The process of wound healing is a dynamic event that starts with inflammation, proliferation, and migration of various types of fibroblast cells and aims to restore the integrity and functionality of damaged tissue. One crucial signaling pathway involved in wound healing is the Wnt signaling pathway. This study aimed to investigate the effects of a cocktail consisting of L-carnosine, zinc sulfate, and B-complex vitamins on the modulation of the Wnt signaling pathway during wound healing. Methods: The optimal dosages of L-carnosine, zinc sulfate, and B-complex were used to prepare the cocktail. Then, the cocktail-mediated wound healing was measured through scratch assay on human dermal fibroblast (HDF) cells. Finally, the effects of the cocktail on the expression of SFRP1, SFRP2, SFRP4, MMP7, and RSPO2 genes involved in the Wnt signaling pathway were measured during the wound closure phenomenon by real-time quantitative polymerase chain reaction (RT-qPCR). Results: The scratch assay demonstrated the wound-healing effects of the prepared cocktail on HDF cells. Additionally, the RT-qPCR results showed that the cocktail can activate the Wnt signaling pathway by down-regulating the expression of SFRP1, SFRP2, SFRP4, and MMP7 and up-regulating the expression of RSPO2. Conclusions: The data suggest that the L-carnosine, zinc sulfate, and B-complex vitamin cocktail exhibits wound healing properties by increasing fibroblast proliferation via the activation of the Wnt pathway.

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调节伤口愈合中的 Wnt 信号通路：左旋肉碱、硫酸锌和复合维生素 B 鸡尾酒的潜力

背景：伤口愈合过程是一个动态过程，首先是各种类型的成纤维细胞发生炎症、增殖和迁移，目的是恢复受损组织的完整性和功能性。参与伤口愈合的一个重要信号通路是 Wnt 信号通路。本研究旨在探讨由左旋肉碱、硫酸锌和复合维生素 B 组成的鸡尾酒对伤口愈合过程中 Wnt 信号通路调节的影响。研究方法采用左旋肉碱、硫酸锌和复合维生素 B 的最佳剂量制备鸡尾酒。然后，通过对人真皮成纤维细胞（HDF）进行划痕试验，测定鸡尾酒介导的伤口愈合。最后，通过实时定量聚合酶链反应（RT-qPCR）测定了鸡尾酒对伤口闭合过程中参与 Wnt 信号通路的 SFRP1、SFRP2、SFRP4、MMP7 和 RSPO2 基因表达的影响。结果显示划痕试验证明了制备的鸡尾酒对 HDF 细胞的伤口愈合作用。此外，RT-qPCR 结果显示鸡尾酒能通过下调 SFRP1、SFRP2、SFRP4 和 MMP7 的表达以及上调 RSPO2 的表达来激活 Wnt 信号通路。结论数据表明，左旋肉碱、硫酸锌和复合维生素 B 鸡尾酒可通过激活 Wnt 通路增加成纤维细胞的增殖，从而具有伤口愈合的特性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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