{"title":"The Effect of The Insecticide Hulk on Some Genetic Characteristics of The Whitefly and The Cucumber Plant That Parasitizes It","authors":"Rania Ghasan Abd, R. Alsugmiany","doi":"10.55544/jrasb.3.1.37","DOIUrl":null,"url":null,"abstract":"Background: Whitefly, Bemisia tabaci is one of the major injurious sucking pests in tropical and subtropical regions. The effect of the insecticide Hulk, which is used to kill the whitefly, was revealed, and its effect on some of its genes and the genes of the cucumber plant that parasitizes it, was revealed.\nMaterials and Methods: The current study was conducted in Kirkuk Governorate. DNA was extracted from whiteflies treated with Hulk pesticides and not treated with pesticides as a control, and DNA was also extracted from cucumber leaves treated with the Hulk pesticide and not treated with the pesticide as a control in order to compare them. detecting the specific primer of gene 28s in the whitefly Bemisia tabaci and Matk gene in the plant Cucumis sativus. Three treatments of each pesticide at three concentrations (25% a quarter lethal, 50% half lethal, 100% lethal). \nResults: The research found that 25% of the pesticide Halk killed 20% of the insects, 50% killed 40%, and 100% killed 70% within 24 hours. Pesticide Hulk found that cucumber plant mutations in the Matk gene and gene 28s had the best insecticidal effects against whitefly Bemisa tabaci.\nConclusions: Overall, results suggest that ethanolic DNA extracts pesticide Hulk is the occurrence of mutations in the Matk gene in the plant Cucumis sativus and gene 28s in the whitefly Bemisia tabaci showed the highest insecticidal effects on whitefly Bemisa tabaci.","PeriodicalId":507877,"journal":{"name":"Journal for Research in Applied Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal for Research in Applied Sciences and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55544/jrasb.3.1.37","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Whitefly, Bemisia tabaci is one of the major injurious sucking pests in tropical and subtropical regions. The effect of the insecticide Hulk, which is used to kill the whitefly, was revealed, and its effect on some of its genes and the genes of the cucumber plant that parasitizes it, was revealed.
Materials and Methods: The current study was conducted in Kirkuk Governorate. DNA was extracted from whiteflies treated with Hulk pesticides and not treated with pesticides as a control, and DNA was also extracted from cucumber leaves treated with the Hulk pesticide and not treated with the pesticide as a control in order to compare them. detecting the specific primer of gene 28s in the whitefly Bemisia tabaci and Matk gene in the plant Cucumis sativus. Three treatments of each pesticide at three concentrations (25% a quarter lethal, 50% half lethal, 100% lethal).
Results: The research found that 25% of the pesticide Halk killed 20% of the insects, 50% killed 40%, and 100% killed 70% within 24 hours. Pesticide Hulk found that cucumber plant mutations in the Matk gene and gene 28s had the best insecticidal effects against whitefly Bemisa tabaci.
Conclusions: Overall, results suggest that ethanolic DNA extracts pesticide Hulk is the occurrence of mutations in the Matk gene in the plant Cucumis sativus and gene 28s in the whitefly Bemisia tabaci showed the highest insecticidal effects on whitefly Bemisa tabaci.
背景: 烟粉虱(Bemisia tabaci)是热带和亚热带地区的主要吸浆害虫之一。本研究揭示了用于杀灭粉虱的杀虫剂 Hulk 的效果,以及它对粉虱基因和寄生粉虱的黄瓜植物基因的影响:本研究在基尔库克省进行。从使用 Hulk 杀虫剂处理过的粉虱和未使用杀虫剂处理过的粉虱身上提取 DNA 作为对照,并从使用 Hulk 杀虫剂处理过的黄瓜叶片和未使用杀虫剂处理过的黄瓜叶片上提取 DNA 作为对照,以便进行比较。每种杀虫剂按三种浓度(25%四分之一致死率、50%半致死率、100%致死率)处理三次。结果 研究发现,在 24 小时内,25% 的农药 Halk 能杀死 20% 的昆虫,50% 能杀死 40%的昆虫,100% 能杀死 70% 的昆虫。农药 Hulk 发现,黄瓜植株的 Matk 基因和 28s 基因突变对烟粉虱的杀虫效果最好:总之,结果表明,乙醇 DNA 提取物杀虫剂 Hulk 是发生突变的植物黄瓜中 Matk 基因和 28s 基因对烟粉虱 Bemisia tabaci 的杀虫效果最好。