Molecular validation of Tomato Yellow Leaf curl Virus (TYLCV) (Ty-1/3, Ty-2 and Ty-3), Fusarium wilt (I-2) and Root- Knot Nematode (Mi-1) resistance in the breeding lines of tomato (Solanum lycopersicum L.)

Q4 Agricultural and Biological Sciences ELECTRONIC JOURNAL OF PLANT BREEDING Pub Date : 2024-03-05 DOI:10.37992/2024.1501.010
Arumugam Nithyanandam, T. Saraswathi, L. Pugalendhi, C. Indu, N. Manivannan, S. Harish, N. Boopathi
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Abstract

Gene based molecular screening for disease resistance has become a prominent and widely adopted approach in contemporary tomato breeding programs, both in the public and private sectors. The effectiveness of disease-resistant molecular screening relies on the selection of suitable markers that span the entire genomic region for accurate identification of resistant alleles. In this study, various co-dominant and dominant Sequence Characterized Amplified Region (SCAR) markers were employed to screen for resistance genes associated with Tomato yellow leaf curl virus (TyLCV) Ty -1, Ty -2, Ty -3, Fusarium wilt (race-2) I-2, and Root-knot Nematode (( Meloidogyne incognita ) Mi -1. The selected markers exhibited high robustness in distinguishing between resistant and susceptible lines. Co-dominant SCAR markers M2 ( Ty -1 gene) and TG0302 ( Ty-2 gene) efficiently identify homozygous or heterozygous alleles in Tomato yellow leaf curl virus (TyLCV) screening. The P6-25 marker for Ty -3 gene discerns Ty -3, Ty -3a, and Ty -3b alleles, enhancing allele specificity. In Fusarium wilt I -2 gene screening, I-2/5 ensures accurate identification, while in Root-knot Nematode Mi -1 gene screening, co-dominant marker Mi-23 reliably identify and distinguishes homozygous or heterozygous resistant lines without false positives for I -2 and Mi -1 genes. This streamlined marker assisted approach optimizes disease resistance screening in tomato breeding, providing valuable insights for disease resistant cultivar development.
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番茄(Solanum lycopersicum L.)育种品系对番茄黄叶卷曲病毒(TYLCV)(Ty-1/3、Ty-2 和 Ty-3)、镰刀菌枯萎病(I-2)和根结线虫(Mi-1)抗性的分子验证
在当代番茄育种计划中,基于基因的抗病性分子筛选已成为公共和私营部门广泛采用的重要方法。抗病分子筛选的有效性依赖于选择合适的标记,这些标记应跨越整个基因组区域,以准确鉴定抗病等位基因。本研究采用了多种共显性和显性序列特征扩增区(SCAR)标记来筛选与番茄黄叶卷曲病毒(TyLCV)Ty-1、Ty-2、Ty-3、镰刀菌枯萎病(race-2)I-2 和根结线虫(Meloidogyne incognita)Mi-1 相关的抗病基因。所选标记在区分抗性品系和易感品系方面表现出较高的稳健性。在番茄黄曲叶病毒(TyLCV)筛选中,共显性 SCAR 标记 M2(Ty-1 基因)和 TG0302(Ty-2 基因)能有效识别同源或杂合等位基因。Ty -3基因的P6-25标记可识别Ty -3、Ty -3a和Ty -3b等位基因,提高了等位基因的特异性。在镰刀菌枯萎病 I -2 基因筛选中,I-2/5 可确保准确鉴定;在根结线虫 Mi -1 基因筛选中,共显性标记 Mi-23 可可靠地鉴定和区分同源或杂源抗性品系,而不会出现 I -2 和 Mi -1 基因的假阳性。这种简化的标记辅助方法优化了番茄育种中的抗病性筛选,为抗病栽培品种的开发提供了宝贵的见解。
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来源期刊
ELECTRONIC JOURNAL OF PLANT BREEDING
ELECTRONIC JOURNAL OF PLANT BREEDING Agricultural and Biological Sciences-Soil Science
CiteScore
1.10
自引率
0.00%
发文量
153
审稿时长
25 weeks
期刊介绍: Electronic Journal of Plant Breeding (EJPB) is an official publication of Indian Socciety of Plant Breeders (ISPB). The main aim of this journal is to promote the general advancement of plant breeding and to create a forum to bring together and facilitate the exchange of information amongst plant breeders. It is published quarterly as ONLINE as OPEN ACCESS journal in the official web page www.ejplantbreeding.org. The publications are subjected into DOUBLE BLIND PEER REVIEWES. Original research articles and research communications on all areas of Plant Breeding viz., genetics, breeding, cytology, germplasm evaluation, marker assisted breeding, etc are accepted. At present Review articles are not accepted for publication.
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