Efficiency of three different fluoride varnishes on enamel remineralization of primary teeth: An in vitro study

Omar Sobhy Asaad, M. Farag, Yousra Helmy
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Abstract

Introduction: Fluoride is fundamental in the prevention of dental caries; it enhances the precipitation of calcium and phosphates, forming fluorapatite, which is more acid-resistant than hydroxyapatite. Recently, Nano Silver Fluoride was presented as a new remineralizing agent. Aim: This study aimed to compare the remineralization potential of three different fluoride varnishes Fluor Protector varnish (FP), Enamel Pro varnish (EP), and Nano Silver Fluoride varnish (NSF) on the enamel of primary teeth. Materials and Methods: 80 samples were gathered, and randomly distributed among three groups: negative control group, demineralized group, and study group which was subdivided into 3 subgroups, subgroup A: Fluor Protector, subgroup B: Enamel Pro and subgroup C: Nano silver fluoride. Pepsi was used for 72 hours to demineralize the Positive control group and the study group. Varnishes were then applied. All teeth were subjected to pH cycle in a demineralization solution/artificial saliva for 10 days. Vicker’s microhardness test, Energy Dispersive X-ray Spectroscopy (EDX), and Environmental Scanning Electron Microscope (ESM) were used to measure the extent of enamel mineralization. The data were then, statistically evaluated. Results: Enamel Pro showed the highest results followed by Fluor protector and Nano silver fluoride however, there was no statistically significant difference between the three tested groups. Conclusion: The study showed that the Enamel Pro had the highest values in microhardness, EDX, and ESM, but there was no significant statistical difference between the three groups. All the materials used proved their efficiency in remineralization of the demineralized enamel.
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三种不同氟化物清漆对乳牙釉质再矿化的作用:体外研究
简介氟是预防龋齿的基本物质;它能促进钙和磷酸盐的沉淀,形成氟磷灰石,而氟磷灰石比羟基磷灰石更耐酸。最近,纳米银氟化物作为一种新的再矿化剂问世。目的:本研究旨在比较三种不同的氟化物清漆--氟保护剂清漆(FP)、珐琅质保护剂清漆(EP)和纳米银氟化物清漆(NSF)对乳牙珐琅质的再矿化潜力。材料与方法:收集 80 份样本,随机分配给三组:阴性对照组、脱矿组和研究组:A 组:氟保护剂;B 组:珐琅质保护剂;C 组:纳米银氟化物:纳米银氟化物。使用百事可乐 72 小时,使阳性对照组和研究组的牙齿脱矿。然后涂上清漆。所有牙齿在脱矿物质溶液/人工唾液中进行 pH 循环 10 天。维氏硬度测试、能量色散 X 射线光谱仪 (EDX) 和环境扫描电子显微镜 (ESM) 被用来测量牙釉质矿化的程度。然后,对数据进行统计评估。结果显示珐琅质保护剂的效果最好,其次是氟保护剂和纳米银氟化物,但三组测试结果在统计学上没有显著差异。结论研究表明,珐琅质保护剂的显微硬度、EDX 和 ESM 值最高,但三组之间没有明显的统计学差异。所有使用的材料都证明了它们在脱矿釉质再矿化方面的功效。
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