C. Rowlands, Ke Guo, P. Pantazis, Konstantinos Kalyviotis
{"title":"Hyperspectral oblique plane microscopy","authors":"C. Rowlands, Ke Guo, P. Pantazis, Konstantinos Kalyviotis","doi":"10.1117/12.3003078","DOIUrl":null,"url":null,"abstract":"A rapid, efficient 3D-resolved hyperspectral microscope is presented. Able to switch between hyperspectral and normal imaging modes in seconds without realignment, it is built around a commercial inverted microscope frame for ease-of-use. Performance is demonstrated on a variety of samples; utility is shown by performing highly-multiplexed fluorescence microscopy, and the sensitivity and resolution is highlighted by rapidly taking spontaneous Raman maps of micro-plastics and zebrafish embryos","PeriodicalId":517893,"journal":{"name":"High-Speed Biomedical Imaging and Spectroscopy IX","volume":"4 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"High-Speed Biomedical Imaging and Spectroscopy IX","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1117/12.3003078","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
A rapid, efficient 3D-resolved hyperspectral microscope is presented. Able to switch between hyperspectral and normal imaging modes in seconds without realignment, it is built around a commercial inverted microscope frame for ease-of-use. Performance is demonstrated on a variety of samples; utility is shown by performing highly-multiplexed fluorescence microscopy, and the sensitivity and resolution is highlighted by rapidly taking spontaneous Raman maps of micro-plastics and zebrafish embryos
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