Integrative methylome and transcriptome analysis reveals epigenetic regulation of Fusobacterium nucleatum in laryngeal cancer.

IF 4 2区生物学 Q1 GENETICS & HEREDITY Microbial Genomics Pub Date : 2024-03-01 DOI:10.1099/mgen.0.001221

Xiaohui Yuan, Hui-Ching Lau, Huiying Huang, Chi-Yao Hsueh, Hongli Gong, Liang Zhou

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Abstract

The aetiological mechanisms of Fusobacterium nucleatum in laryngeal cancer remain unclear. This study aimed to reveal the epigenetic signature induced by F. nucleatum in laryngeal squamous cell carcinoma (LSCC). Combined analysis of methylome and transcriptome data was performed to address the functional role of F. nucleatum in laryngeal cancer. Twenty-nine differentially expressed methylation-driven genes were identified by mapping the methylation levels of significant differential methylation sites to the expression levels of related genes. The combined analysis revealed that F. nucleatum promoted Janus kinase 3 (JAK3) gene expression in LSCC. Further validation found decreased methylation and elevated expression of JAK3 in the F. nucleatum-treated LSCC cell group; F. nucleatum abundance and JAK3 gene expression had a positive correlation in tumour tissues. This analysis provides a novel understanding of the impact of F. nucleatum in the methylome and transcriptome of laryngeal cancer. Identification of these epigenetic regulatory mechanisms opens up new avenues for mechanistic studies to explore novel therapeutic strategies.

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甲基组和转录组的整合分析揭示了核酸分枝杆菌在喉癌中的表观遗传调控。

核叉杆菌在喉癌中的致病机制尚不清楚。本研究旨在揭示核酸桿菌在喉鳞状细胞癌（LSCC）中诱导的表观遗传学特征。研究人员对甲基组和转录组数据进行了联合分析，以探讨核酸酵母菌在喉癌中的功能作用。通过将显著差异甲基化位点的甲基化水平与相关基因的表达水平进行映射，确定了 29 个差异表达的甲基化驱动基因。综合分析表明，F. nucleatum 促进了 LSCC 中 Janus 激酶 3 (JAK3) 基因的表达。进一步验证发现，在经 F. nucleatum 处理的 LSCC 细胞组中，JAK3 的甲基化水平降低，表达水平升高；在肿瘤组织中，F. nucleatum 的丰度与 JAK3 基因的表达呈正相关。这项分析提供了一种新的理解，即F. nucleatum对喉癌甲基组和转录组的影响。这些表观遗传调控机制的确定为机理研究探索新的治疗策略开辟了新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial Genomics Medicine-Epidemiology

CiteScore

6.60

自引率

2.60%

发文量

153

审稿时长

12 weeks

期刊介绍： Microbial Genomics (MGen) is a fully open access, mandatory open data and peer-reviewed journal publishing high-profile original research on archaea, bacteria, microbial eukaryotes and viruses.