{"title":"Measurement of the Kinetics of Chlorophyll a Fluorescence by an LED-Light Source Fluorimeter, Handy PEA.","authors":"Marta Hornyák, Agnieszka Płażek","doi":"10.1007/978-1-0716-3794-4_13","DOIUrl":null,"url":null,"abstract":"<p><p>The chlorophyll a fluorescence measurement method is used to determine the efficiency of the photosynthetic apparatus and to assess the physiological state of photosynthetic organisms. The measurement is simple, fast, and noninvasive. It is a precise tool to study photosynthesis response under stress conditions or to assess the impact of specific environmental factors on plants. Here we describe the usage of this method in environmental-controlled plant production systems differing in temperature or light source on the growth and development of common buckwheat.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/978-1-0716-3794-4_13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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Abstract
The chlorophyll a fluorescence measurement method is used to determine the efficiency of the photosynthetic apparatus and to assess the physiological state of photosynthetic organisms. The measurement is simple, fast, and noninvasive. It is a precise tool to study photosynthesis response under stress conditions or to assess the impact of specific environmental factors on plants. Here we describe the usage of this method in environmental-controlled plant production systems differing in temperature or light source on the growth and development of common buckwheat.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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