The Complete Genome Sequence of Bacillus toyonensis Cbmb3 with Polyvinyl Chloride-Degrading Properties.

IF 6.8 Q1 TOXICOLOGY Journal of Xenobiotics Pub Date : 2024-02-26 DOI:10.3390/jox14010018

Dandan Wang, Hong Yu, Xinbei Liu, Li Sun, Xijian Liu, Ruilong Hu, Chao Wang, Yuping Zhuge, Zhihong Xie

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Abstract

The accumulation of high amounts of plastic waste in the environment has raised ecological and health concerns, particularly in croplands, and biological degradation presents a promising approach for the sustainable treatment of this issue. In this study, a polyvinyl chloride (PVC)-degrading bacterium was isolated from farmland soil samples attached to waste plastic, utilizing PVC as the sole carbon source. The circular chromosome of the strain Cbmb3, with a length of 5,768,926 bp, was subsequently sequenced. The average GC content was determined to be 35.45%, and a total of 5835 open reading frames were identified. The strain Cbmb3 was designated as Bacillus toyonensis based on phylogenomic analyses and genomic characteristics. The bioinformatic analysis of the Cbmb3 genome revealed putative genes encoding essential enzymes involved in PVC degradation. Additionally, the potential genomic characteristics associated with phytoprobiotic effects, such as the synthesis of indole acetic acid and secondary metabolite synthesis, were also revealed. Overall, the present study provides the first complete genome of Bacillus toyonensis with PVC-degrading properties, suggesting that Cbmb3 is a potential strain for PVC bioremediation and application.

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具有聚氯乙烯降解特性的丰饶芽孢杆菌 Cbmb3 的完整基因组序列。

大量塑料垃圾在环境中的积累引发了生态和健康问题，尤其是在农田中。本研究从附着在废塑料上的农田土壤样本中分离出一种聚氯乙烯（PVC）降解细菌，该细菌以聚氯乙烯为唯一碳源。随后对菌株 Cbmb3 的环状染色体进行了测序，其长度为 5,768,926 bp。平均 GC 含量为 35.45%，共鉴定出 5835 个开放阅读框。根据系统发生组分析和基因组特征，将菌株 Cbmb3 定名为玩具诺氏芽孢杆菌（Bacillus toyonensis）。对 Cbmb3 基因组进行的生物信息学分析揭示了编码参与聚氯乙烯降解的重要酶的推定基因。此外，还揭示了与植物生物效应相关的潜在基因组特征，如吲哚乙酸的合成和次生代谢物的合成。总之，本研究首次提供了具有聚氯乙烯降解特性的丰产芽孢杆菌（Bacillus toyonensis）的完整基因组，表明 Cbmb3 是聚氯乙烯生物修复和应用的潜在菌株。

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来源期刊

Journal of Xenobiotics TOXICOLOGY-

CiteScore

5.30

自引率

1.70%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Xenobiotics publishes original studies concerning the beneficial (pharmacology) and detrimental effects (toxicology) of xenobiotics in all organisms. A xenobiotic (“stranger to life”) is defined as a chemical that is not usually found at significant concentrations or expected to reside for long periods in organisms. In addition to man-made chemicals, natural products could also be of interest if they have potent biological properties, special medicinal properties or that a given organism is at risk of exposure in the environment. Topics dealing with abiotic- and biotic-based transformations in various media (xenobiochemistry) and environmental toxicology are also of interest. Areas of interests include the identification of key physical and chemical properties of molecules that predict biological effects and persistence in the environment; the molecular mode of action of xenobiotics; biochemical and physiological interactions leading to change in organism health; pathophysiological interactions of natural and synthetic chemicals; development of biochemical indicators including new “-omics” approaches to identify biomarkers of exposure or effects for xenobiotics.