Bart Weijts, Eugene Tkachenko, David Traver, Alex Groisman
{"title":"A Four-Well Dish for High-Resolution Longitudinal Imaging of the Tail and Posterior Trunk of Larval Zebrafish.","authors":"Bart Weijts, Eugene Tkachenko, David Traver, Alex Groisman","doi":"10.1089/zeb.2016.1406","DOIUrl":null,"url":null,"abstract":"<p><p>We describe the design, fabrication, and applications of a four-well dish for imaging of the trunk of larval zebrafish. The dish facilitates immobilization of anesthetized zebrafish larvae, with their tails gently pushed against a microscope cover glass, enabling longitudinal imaging at 24-72 h postfertilization using high-resolution objective lenses.</p>","PeriodicalId":94273,"journal":{"name":"Zebrafish","volume":"14 5","pages":"489-491"},"PeriodicalIF":0.0000,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650709/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zebrafish","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/zeb.2016.1406","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/1/24 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
We describe the design, fabrication, and applications of a four-well dish for imaging of the trunk of larval zebrafish. The dish facilitates immobilization of anesthetized zebrafish larvae, with their tails gently pushed against a microscope cover glass, enabling longitudinal imaging at 24-72 h postfertilization using high-resolution objective lenses.
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