Effects of stearic acid on the embryo cryopreservation in mouse.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-01-01

T N Igonina, T A Rakhmanova, A N Omelchenko, K A Okotrub, E Yu Brusentsev, I N Rozhkova, S Ya Amstislavsky

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Abstract

Background: Intracellular lipids are sensitive to freezing. Lipidome modification is an important tool for studying the role of intracellular lipids in cryotolerance of mammalian oocytes and preimplantation embryos.

Objective: To study the effects of in vitro exposure of murine embryos to saturated stearic acid (SA) on the lipid content, embryo development and cryotolerance.

Materials and methods: In vivo derived mouse embryos were cultured with 100 uM SA for 48 h up to the morula/blastocyst stage. Some of the SA-treated embryos were chosen for the evaluation of their development competence and the change in the lipidome, and other embryos were either slowly frozen or rapidly vitrified.

Results: Nile red staining combined with confocal laser scanning microscopy revealed a decrease in the total amount of lipids in the SA-treated embryos. Raman measurements showed that the lipid unsaturation was lower in embryos after in vitro SA culture. The addition of SA did not affect the embryo development before cryopreservation, but negatively affected the results of slow freezing cryopreservation and vitrification.

Conclusion: In vitro SA exposure lowered the total amount of intracellular lipids and unsaturation in mouse embryos. The changes were accompanied with a significantly lower efficacy of embryo cryopreservation. https://doi.org/10.54680/fr24110110512.

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硬脂酸对小鼠胚胎冷冻的影响

背景：细胞内脂质对冷冻敏感。脂质体修饰是研究细胞内脂质在哺乳动物卵母细胞和植入前胚胎耐冷冻性中的作用的重要工具：研究小鼠胚胎体外暴露于饱和硬脂酸（SA）对脂质含量、胚胎发育和低温耐受性的影响：将体内衍生的小鼠胚胎用 100 uM SA 培养 48 小时，直至胚泡/囊胚期。选择部分经 SA 处理的胚胎评估其发育能力和脂质体的变化，其他胚胎则被缓慢冷冻或快速玻璃化：结果：尼罗河红染色结合共焦激光扫描显微镜显示，经 SA 处理的胚胎中的脂质总量有所减少。拉曼测量显示，体外 SA 培养后的胚胎脂质不饱和度较低。添加 SA 不会影响冷冻保存前的胚胎发育，但会对慢冻冷冻和玻璃化的结果产生负面影响：结论：体外 SA 暴露降低了小鼠胚胎细胞内脂类的总量和不饱和度。https://doi.org/10.54680/fr24110110512。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.