Reduced sperm number of murrah (Bubalus bubalis) bull semen in french mini-straw affects kinetic and functional competence after cryopreservation.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-01-01

H P Yadav, T K Mohanty, R K Dewry, S A Lone, S Nath, M Bhakat, R K Baithalu, S Tiwari, D K Swain, P Kumar, A K Mohanty, T K Datta

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Abstract

Background: Extensive dilution of cattle semen with tris-based extender compromises certain sperm kinetic and functional traits following cryopreservation.

Objective: To study sperm functions of buffalo bulls under high dilution rates.

Materials and methods: Twenty-four ejaculates were harvested twice a week from four buffalo bulls, and diluted to sperm concentrations of 80, 60, 40 and 20 million/mL. Diluted samples were filled in straws, equilibrated at refrigeration temperature for 4 h, and frozen in liquid nitrogen. Frozen sperm samples were thawed for evaluation of kinetic and functional attributes.

Results: Compared to 20 million/mL (million/mL) sperm sample, the total motility, progressive motility and rapid motility were reduced (P < 0.05) in 5 million/mL sample. The proportion of live sperm were significantly (P < 0.05) higher in 10, 15 and 20 million/mL samples than in 5 million/mL sample. The percentage of moribund sperm, dead sperm, and sperm with lipid per oxidation increased significantly (P < 0.05) in 5 million/mL sample.

Conclusion: The reduction of sperm concentrations to < 10 million/mL affects post-thaw Buffalo sperm kinetic and functional attributes.. https://doi.org/10.54680/fr24110110712.

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法式小吸管中布拉氏公牛精液的精子数量减少会影响冷冻保存后的动力学和功能能力。

背景：用三羟甲基丙烷为基础的扩增剂对牛精液进行大范围稀释会损害精子冷冻保存后的某些动力学和功能特性：研究高稀释率下水牛公牛的精子功能：每周两次从四头水牛公牛身上采集24次射精，并稀释至精子浓度分别为8000万、6000万、4000万和2000万/毫升。稀释后的样本装入吸管，在冷藏温度下平衡 4 小时，然后冷冻在液氮中。解冻冷冻精子样本以评估动力学和功能属性：与 2000 万/毫升（百万/毫升）精子样本相比，500 万/毫升样本的总活力、渐进活力和快速活力均有所下降（P < 0.05）。活精子的比例在 1000 万、1500 万和 2000 万/毫升样本中明显高于 500 万/毫升样本（P < 0.05）。在 500 万/毫升样本中，畸形精子、死精子和氧化脂质精子的比例明显增加（P < 0.05）：结论：精子浓度降低到小于 1000 万/毫升会影响解冻后水牛精子的动力学和功能属性。https://doi.org/10.54680/fr24110110712。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.