Hypoxic environment promotes angiogenesis and bone bridge formation by activating Notch/RBPJ signaling pathway in HUVECs

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Genomics Pub Date : 2024-03-25 DOI:10.1016/j.ygeno.2024.110838

Wendong Liu , Mincheng Zou , Mimi Chen , Zheng Zhang , Yunpeng Mao , Yuhao Yang , Ya Liu , Qin Shi , Xiaodong Wang , Fuyong Zhang

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Abstract

After epiphyseal fracture, the epiphyseal plate is prone to ischemia and hypoxia, leading to the formation of bone bridge and deformity. However, the exact mechanism controlling the bone bridge formation remains unclear. Notch/RBPJ signaling axis has been indicated to regulate angiogenesis and osteogenic differentiation. Our study aims to investigate the mechanism of bone bridge formation after epiphyseal plate injury, and to provide a theoretical basis for new therapeutic approaches to prevent the bone bridge formation. The expression of DLL4 and RBPJ was significantly up-regulated in HUVECs after ischemia and hypoxia treatment. Notch/RBPJ pathway positively regulated the osteogenic differentiation of BMSCs. HUVECs can induce osteogenic differentiation of BMSCs under ischemia and hypoxia. Notch/RBPJ pathway is involved in the regulation of the trans-epiphyseal bridge formation. Notch/RBPJ in HUVECs is associated with osteogenic differentiation of BMSCs and may participate in the regulation of the bone bridge formation across the epiphyseal plate.

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缺氧环境通过激活 HUVEC 的 Notch/RBPJ 信号通路促进血管生成和骨桥形成。

骺板骨折后容易缺血缺氧，导致骨桥形成和畸形。然而，控制骨桥形成的确切机制仍不清楚。有研究表明，Notch/RBPJ 信号轴可调控血管生成和成骨分化。我们的研究旨在探讨骺板损伤后骨桥形成的机制，并为预防骨桥形成的新治疗方法提供理论依据。缺血缺氧处理后，HUVECs 中 DLL4 和 RBPJ 的表达明显上调。Notch/RBPJ通路正向调控BMSCs的成骨分化。缺血缺氧条件下，HUVECs 可诱导 BMSCs 成骨分化。Notch/RBPJ通路参与调控跨骺桥的形成。HUVECs中的Notch/RBPJ与BMSCs的成骨分化有关，可能参与了跨骺板骨桥形成的调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.