Isolation and Characterization of an Antioxidant Aryl Polyene Pigment from Antarctic Bacterium Lysobacter sp. A03.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-03-28 DOI:10.1007/s12033-024-01132-7

Maria Elisa Pailliè-Jiménez, Paolo Stincone, Jamile Queiroz Pereira, Patricio Román Santagapita, Eliseu Rodrigues, Adriano Brandelli

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Abstract

Lysobacter is known as a bacterial genus with biotechnological potential, producing an array of enzymes, antimicrobial metabolites, and bioactive antioxidant compounds, including aryl polyene (APE) pigments that have been described as protecting substances against photooxidative damage and lipid peroxidation. In this study, the pigment extracted from keratinolytic Lysobacter sp. A03 isolated from Antarctic environment was characterized. The results of KOH test, UV-vis spectroscopy, CIELAB color system, ¹H-NMR, and FTIR-ATR spectroscopy suggest the pigment is a yellow xanthomonadin-like pigment. The in vitro antioxidant activity of the pigment was confirmed by the scavenging of ABTS and DPPH radicals. In silico analysis of the genome through antiSMASH software was also performed and the secondary metabolite gene clusters for APE and resorcinol synthesis were identified, suggesting that proteins responsible for the pigment biosynthesis are encoded in Lysobacter A03 genome.

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从南极细菌 Lysobacter sp.

溶菌是众所周知的具有生物技术潜力的细菌属，能产生一系列酶、抗菌代谢物和生物活性抗氧化化合物，包括芳基多烯（APE）色素，这些色素被描述为防止光氧化损伤和脂质过氧化的保护物质。本研究对从南极环境中分离的角质溶解性溶菌 A03 提取的色素进行了表征。KOH试验、紫外-可见光谱、CIELAB颜色系统、1H-NMR和傅立叶变换红外-ATR光谱的结果表明，该色素为黄色黄单胞菌素样色素。通过清除 ABTS 和 DPPH 自由基，证实了该色素的体外抗氧化活性。此外，还通过 antiSMASH 软件对基因组进行了硅学分析，发现了合成 APE 和间苯二酚的次级代谢物基因簇，表明溶菌 A03 基因组中编码了负责色素生物合成的蛋白质。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.