Metabolomics Reveals the Impact of Zeolitic Imidazolate Framework-8 on Escherichia coli

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-03-27 DOI:10.1134/s0003683824700029

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Abstract

The increasing use of metal organic frameworks can have many unintended consequences for ecosystems and the environment. However, knowledge of their toxicological behavior and mechanisms of action within the environmental microbial community is extremely limited. In this study, biological and metabolic responses of Escherichia coli to zeolitic imidazolate framework-8 (ZIF-8) were investigated. The results revealed that ZIF-8 could bind to and accumulate on the surface of E. coli cells. Low concentrations of ZIF-8 had little effects on the metabolism of E. coli, while exposure to high-dosage ZIF-8 led to perturbation of cellular functions, including changes of cell membrane permeability, leading to a shortage of energy supply. Moreover, the high-dosage ZIF-8 treatment induced stress-related biological pathways, such as increased metabolism of amino acids, sugars and sugar alcohols. All of these changes resulted in poor growth and low viability of E. coli. Taken together, the results provide detailed picture of the metabolic changes induced by ZIF-8 and are beneficial for assessing the toxicity of ZIF-8 and their further applications.

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代谢组学揭示沸石咪唑酸盐框架-8 对大肠杆菌的影响

摘要越来越多地使用金属有机框架会给生态系统和环境带来许多意想不到的后果。然而，人们对金属有机框架在环境微生物群落中的毒理学行为和作用机制的了解极为有限。本研究调查了大肠杆菌对沸石咪唑啉框架-8（ZIF-8）的生物和代谢反应。结果表明，ZIF-8 可与大肠杆菌细胞结合并在其表面积聚。低浓度的 ZIF-8 对大肠杆菌的新陈代谢影响不大，而接触高剂量的 ZIF-8 则会导致细胞功能紊乱，包括细胞膜通透性发生变化，从而导致能量供应不足。此外，大剂量 ZIF-8 处理诱导了与应激有关的生物通路，如氨基酸、糖和糖醇的代谢增加。所有这些变化都导致大肠杆菌生长不良和活力低下。综上所述，这些结果提供了 ZIF-8 诱导的代谢变化的详细情况，有利于评估 ZIF-8 的毒性及其进一步应用。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.