M. Olenchuk, T. Hanulia, A. Nikolenko, G. Dovbeshko
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引用次数: 0
Abstract
The study of large biomolecules, in particular DNA, is of practical interest. Marker bands of the Raman spectrum of DNA provide information about the conformational state and structure of the macromolecule. At the same time, when dealing with biological experiments, a small amount of DNA is available, which is why it is important to enhance the signal from DNA and find the proper substrate. However, it is difficult to enhance Raman spectra for large molecules as DNA without functionalization that could introduce changes in the DNA structure. In this work, a conventional substrate as CaF2 and a photonic crystal as a substrate with enhancer signal property and without DNA functionalization were applied. The factor of enhancement for DNA from salmon testes was reached up to 10 without Raman spectra distortion. The Raman spectral bands on different types of substrates are analyzed and compared.
研究大型生物大分子，尤其是 DNA，具有实际意义。DNA 拉曼光谱的标记带提供了有关大分子构象状态和结构的信息。与此同时，在进行生物实验时，可用的 DNA 数量很少，因此增强 DNA 信号并找到合适的底物非常重要。然而，要增强 DNA 这种大分子的拉曼光谱，如果不进行功能化处理，DNA 的结构就会发生变化，这一点非常困难。在这项工作中，应用了 CaF2 这种传统基底和光子晶体这种具有增强信号特性且未进行 DNA 功能化的基底。来自鲑鱼睾丸的 DNA 的增强因子高达 10，而拉曼光谱没有发生扭曲。对不同类型基底上的拉曼光谱带进行了分析和比较。
期刊介绍：
Guided by an international editorial board, Low Temperature Physics (LTP) communicates the results of important experimental and theoretical studies conducted at low temperatures. LTP offers key work in such areas as superconductivity, magnetism, lattice dynamics, quantum liquids and crystals, cryocrystals, low-dimensional and disordered systems, electronic properties of normal metals and alloys, and critical phenomena. The journal publishes original articles on new experimental and theoretical results as well as review articles, brief communications, memoirs, and biographies.
Low Temperature Physics, a translation of the copyrighted Journal FIZIKA NIZKIKH TEMPERATUR, is a monthly journal containing English reports of current research in the field of the low temperature physics. The translation began with the 1975 issues. One volume is published annually beginning with the January issues.
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