Association of CovRS two-component regulatory system with NADase induction by Clindamycin treatment in Streptococcus pyogenes

IF 1.1 4区医学 Q4 INFECTIOUS DISEASES Japanese journal of infectious diseases Pub Date : 2024-03-29 DOI:10.7883/yoken.jjid.2023.451

Ichiro Tatsuno, Masanori Isaka, Tadao Hasegawa

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Abstract

The administration of high-dose clindamycin (CLI) along with penicillin is recommended for the treatment of streptococcal toxic shock syndrome (STSS). However, CLI-resistant strains have been identified worldwide. Firstly, in this study, some CLI-resistant strains showed increased extracellular activities of the NAD- glycohydrolase (NADase) exotoxin after CLI treatment. This result supported our previous conclusion that not only CLI-susceptible but also CLI-resistant S. pyogenes strains show the CLI-dependent NADase induction. Secondary, using the 13 types of two- component-sensor knockout strains derived from a CLI-susceptible strain 1529 that has the CLI-dependent NADase induction phenotype, we investigated the mechanism of action. Among the knockout strains, only 1529ΔcovS lost the phenotype. In addition, 1529ΔspeB, 1529Δmga, and 1529Δrgg retained the CLI-dependent NADase induction phenotype. These results suggest that CovS is related to the phenotype in SpeB independent manner.

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化脓性链球菌中 CovRS 双组分调控系统与克林霉素处理诱导 NAD 酶的关系

在治疗链球菌中毒性休克综合征（STSS）时，建议使用大剂量克林霉素（CLI）和青霉素。然而，世界各地都发现了耐克林霉素的菌株。首先，在本研究中，一些对 CLI 耐药的菌株在接受 CLI 治疗后，NAD-糖水解酶（NADase）外毒素的胞外活性增加。这一结果支持了我们之前的结论，即不仅对 CLI 易感的化脓性链球菌菌株，而且对 CLI 耐药的化脓性链球菌菌株也表现出依赖 CLI 的 NAD 酶诱导。其次，我们利用从具有 CLI 依赖性 NAD 酶诱导表型的 CLI 易感菌株 1529 衍生出的 13 种双组分传感器基因敲除菌株，研究了其作用机制。在这些基因敲除菌株中，只有 1529ΔcovS 失去了这种表型。此外，1529ΔspeB、1529Δmga 和 1529Δrgg 保留了 CLI 依赖性 NADase 诱导表型。这些结果表明，CovS 以独立于 SpeB 的方式与表型相关。

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来源期刊

Japanese journal of infectious diseases 医学-传染病学

CiteScore

4.50

自引率

4.50%

发文量

172

审稿时长

2 months

期刊介绍： Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.