Detection of abnormal behaviors in prenatal Poly(I:C) exposed mice in a group-rearing environment

IF 1.3 4区 医学 Q3 PEDIATRICS Congenital Anomalies Pub Date : 2024-03-31 DOI:10.1111/cga.12563
Munekazu Komada, Niina Kiriyama, Rei Sugiyama, Kazuma Harada, Norihito Kawashita
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Abstract

During pregnancy, the maternal environment is critical for normal ontogeny and central nervous system development. Occasionally, prenatal exposure to environmental factors affects tissue architecture and functional development of the brain, which causes developmental disorders, including disorders of the autism spectrum. One of these environmental factors is the exposure to infectious diseases during pregnancy. In this study, we generated mice with infectious disease-induced inflammation by prenatal exposure to 200 μg/kg polyinosinic–polycytidylic acid sodium salt [Poly(I:C)] at embryonic day 12.5 and analyzed their phenotypes on 30-weeks-old. We attempted to detect abnormalities in spontaneous activity and social interaction, which may be indicators of developmental disorder-like behavioral abnormalities, in free-ranging behaviors in multiple rearing environments using multiple animal positioning systems and UMATracker in mice with fetal inflammation. Increased spontaneous activity and abnormal social interactions were observed in mice in the Poly(I:C)-treated group compared with those in the control group. Prenatal exposure to Poly(I:C) increased motor activity and decreased social interaction, and social behavior in prenatally treated mice in a multiple-individual rearing environment. Poly(I:C) exposure during the fetal period resulted in developmental disorder-like behavioral abnormalities, such as increased activity and abnormal social interactions, even after maturation in a multiple-individual rearing environment. This experimental method may provide a new way to analyze the behavior of mouse models of developmental disorders in a multiple-individual rearing environment, in which free-ranging behavior is possible.

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在群养环境中检测产前暴露于聚(I:C)的小鼠的异常行为。
怀孕期间,母体环境对正常的本体发育和中枢神经系统发育至关重要。偶尔,产前暴露于环境因素会影响大脑的组织结构和功能发育,从而导致发育障碍,包括自闭症谱系障碍。这些环境因素之一就是孕期接触传染性疾病。在这项研究中,我们通过在胚胎12.5天时产前暴露于200微克/千克的聚肌苷-聚胞苷酸钠盐[Poly(I:C)],产生了感染性疾病诱导的炎症小鼠,并分析了它们在30周大时的表型。我们试图利用多种动物定位系统和 UMATracker 检测胎儿炎症小鼠在多种饲养环境中自由活动时的自发活动和社会交往异常,这可能是类似发育障碍的行为异常的指标。与对照组相比,Poly(I:C)处理组小鼠的自发活动增加,社会交往异常。在多个体饲养环境中,产前暴露于Poly(I:C)的小鼠运动活动增加,社会互动和社会行为减少。胎儿期接触聚(I:C)会导致类似发育障碍的行为异常,如活动增加和社会交往异常,即使在多个体饲养环境中成熟后也是如此。这种实验方法可能为在多个体饲养环境中分析发育障碍小鼠模型的行为提供了一种新的途径,在这种环境中,小鼠的自由活动行为是可能的。
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来源期刊
Congenital Anomalies
Congenital Anomalies PEDIATRICS-
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Congenital Anomalies is the official English language journal of the Japanese Teratology Society, and publishes original articles in laboratory as well as clinical research in all areas of abnormal development and related fields, from all over the world. Although contributions by members of the teratology societies affiliated with The International Federation of Teratology Societies are given priority, contributions from non-members are welcomed.
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