Linear dichroism reveals the perpendicular orientation of DNA bases in the RecA and Rad51 recombinase filaments: A possible mechanism for the strand exchange reaction
Masayuki Takahashi, Kentaro Ito, Hiroshi Iwasaki, Bengt Norden
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Abstract
Linear dichroism spectroscopy is used to investigate the structure of RecA family recombinase filaments (RecA and Rad51 proteins) with DNA for clarifying the molecular mechanism of DNA strand exchange promoted by these proteins and its activation. The measurements show that the recombinases promote the perpendicular base orientation of single-stranded DNA only in the presence of activators, indicating the importance of base orientation in the reaction. We summarize the results and discuss the role of DNA base orientation.
线性二色性显示了 RecA 和 Rad51 重组酶丝中 DNA 碱基的垂直取向：链交换反应的可能机制。
线性分色光谱法用于研究 RecA 家族重组酶丝（RecA 和 Rad51 蛋白）与 DNA 的结构，以阐明这些蛋白促进 DNA 链交换及其激活的分子机制。测量结果表明，只有在激活剂存在的情况下，重组酶才能促进单链 DNA 的垂直碱基取向，这表明碱基取向在反应中的重要性。我们对结果进行了总结，并讨论了 DNA 碱基取向的作用。
期刊介绍：
The main aim of the journal is to publish original contributions of scientific work on the role of chirality in chemistry and biochemistry in respect to biological, chemical, materials, pharmacological, spectroscopic and physical properties.
Papers on the chemistry (physiochemical, preparative synthetic, and analytical), physics, pharmacology, clinical pharmacology, toxicology, and other biological aspects of chiral molecules will be published.
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