A novel vitrified cryopreservation approach with stem cell-laden hydrogel microcapsules.

IF 1 4区 生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-03-01
T Song, B Liu
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引用次数: 0

Abstract

Background: Stem cell-laden hydrogel microcapsules construction is important for a wide application in tissue engineering and cell-based medicine, such as building an ideal immune barrier. Challenges are emerging for effectively storing such microcapsules by cryopreservation, and a large proportion of research has been on the cryopreservation of single cells encapsulated into microcapsules without a core-shell structure.

Objective: To achieve the effective cryopreservation of stem cell-laden hydrogel microcapsules with a core-shell structure.

Materials and methods: A novel core-shell alginate hydrogel encapsulation method was used to produce mesenchymal stem cell-laden microcapsules by microfluidic technique.

Results: This microcapsule could inhibit ice formation to achieve vitreous cryopreservation with a low concentration (2 M) of penetrating cryoprotectants.

Conclusion: Cell laden hydrogel microcapsules may have the potential to be the basis of a new strategy of cell cryopreservation and applications. https://doi.org/10.54680/fr24210110212.

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使用干细胞水凝胶微囊的新型玻璃化冷冻保存方法。
背景:干细胞水凝胶微胶囊的构建对于组织工程和细胞医学的广泛应用非常重要,例如构建理想的免疫屏障。通过低温冷冻有效保存此类微胶囊的挑战正在出现,而大部分研究都是针对封装在无核壳结构微胶囊中的单个细胞的低温冷冻:材料与方法:采用一种新型的核壳海藻酸盐水凝胶封装方法,通过微流控技术制备出含有间充质干细胞的微胶囊:结果:这种微胶囊可以抑制冰的形成,从而在低浓度(2 M)渗透性低温保护剂的作用下实现玻璃体低温保存:细胞载体水凝胶微胶囊有可能成为细胞冷冻保存和应用新策略的基础。https://doi.org/10.54680/fr24210110212。
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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