Optimizing semen cryopreservation in Calomys laucha: a step forward in rodent reproductive research.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-03-01

C D Corcini, J Vilela, N Gatti, B Mion, N Castro, R T Franca, A S Varela Junior

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引用次数: 0

Abstract

Background: Examining semen cryopreservation in Calomys laucha offers valuable insights for reproductive research and species conservation.

Objective: To determine the most effective sugar for the cryopreservation of C. laucha semen.

Materials and methods: Using 36 epididymides from C. laucha, semen samples were diluted in a 3% skimmed milk medium supplemented with one of four sugars (glucose, fructose, lactose, or sucrose) at a concentration of 0.3 M. These mixtures underwent a conditioning phase at 37 degree C for 10 min, cooled to -80 degree C for another 10 min, and were subsequently stored in liquid nitrogen.

Results: Upon thawing, samples treated with lactose and glucose solutions show superior sperm motility, achieving 8.2% and 10.0% respectively, in contrast to the fructose (2.0%) and sucrose (4.1%) mixtures. Furthermore, samples preserved in glucose registered the highest sperm penetration rates, reaching 44.9%.

Conclusion: Our findings suggest that a cryopreservation medium containing 0.3 M glucose can contribute to the safeguarding C. laucha rodent semen. https://doi.org/10.54680/fr24210110612.

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优化 Calomys laucha 的精液冷冻保存：啮齿动物生殖研究的一大进步。

背景：研究Calomys laucha的精液冷冻保存为生殖研究和物种保护提供了有价值的见解：目的：确定冷冻保存乌贼精液最有效的糖：精液样本在 3% 脱脂奶培养基中稀释，培养基中添加浓度为 0.3 M 的四种糖（葡萄糖、果糖、乳糖或蔗糖）之一：解冻后，经乳糖和葡萄糖溶液处理的样本精子活力更强，分别达到 8.2% 和 10.0%，与果糖（2.0%）和蔗糖（4.1%）混合物形成鲜明对比。此外，在葡萄糖中保存的样本精子穿透率最高，达到 44.9%：https://doi.org/10.54680/fr24210110612。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.