RNA-Seq analysis reveals the mechanism in response to cold stress of peach cv 'Dingjiaba'.

IF 1 4区 生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-03-01
X Li, H Wang, X Wu
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Abstract

Background: 'Dingjiaba' is an important Prunus persica cultivar (cv) mainly grown in the Hexi corridor in northwest China, which has an inherited strong cold tolerance.

Objective: To compare the transcriptome and physiology data of leaves of cvs 'Dingjiaba' (D) and 'Kanoiwa' (K) following cold treatment at different time periods, in order to gain new insights into the mechanisms of cold adaptation in 'Dingjiaba'.

Materials and methods: We analyzed the transcriptomic and physiological data of leaves of D and K cvs exposed to 0 h (D0/K0), 2 h (D2/K2), 6 h (D6/K6) and 12 h (D12/K12) cold stress.

Results: Low temperature stress caused membrane damage and led to increased rate of electrolyte leakage and increased MDA content. Cold stress induced the accumulation of soluble sugars, soluble proteins and proline in leaves of both cvs, with a lower increase in K compared to D. Transcriptome analysis identified 4,631, 5,069, 5,662 and 3,886 differentially expressed genes (DEGs) between D0 and K0, D2 and K2, D6 and K6 and D12 and K12, respectively. The differentially expressed genes significantly enriched in metabolic pathways and biosynthesis of secondary metabolites. We further validated the reliability of sequencing data of the RNA-Seq with Real-Time Quantitative PCR, which suggested that the expression trend of the RNA-Seq were same as RT-PCR.

Conclusions: These results provide novel insights into a series of molecular mechanisms underlying physiological metabolism and defense. https://doi.org/10.54680/fr24210110312.

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RNA-Seq 分析揭示了桃品种 "丁家坝 "对冷胁迫的响应机制。
背景:'丁家坝'是一个重要的柿树栽培品种(cv),主要生长在中国西北部的河西走廊,具有遗传性强的耐寒性:目的:比较 "丁家坝"(D)和 "香乃岩"(K)在不同时期经冷处理后叶片的转录组和生理数据,以期对 "丁家坝 "的冷适应机制有新的认识:我们分析了暴露于 0 小时(D0/K0)、2 小时(D2/K2)、6 小时(D6/K6)和 12 小时(D12/K12)低温胁迫的 D 和 K 品种叶片的转录组和生理数据:低温胁迫造成膜损伤,导致电解质渗漏率增加和 MDA 含量增加。转录组分析在 D0 和 K0、D2 和 K2、D6 和 K6 以及 D12 和 K12 之间分别发现了 4 631、5 069、5 662 和 3 886 个差异表达基因(DEGs)。差异表达基因明显富集于代谢途径和次生代谢物的生物合成。我们进一步用实时定量 PCR 验证了 RNA-Seq 测序数据的可靠性,结果表明 RNA-Seq 的表达趋势与 RT-PCR 相同:https://doi.org/10.54680/fr24210110312。
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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