Testing of arctic insect hemolymph as a secondary agent in applied cryopreservation.

IF 1 4区 生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-03-01
N G Li
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Abstract

Background: Cold hardiness of insects from extremely cold regions is based on a principle of natural cryoprotection, which is associated with physiological mechanisms provided by cryoprotectants.

Objective: Since arctic cold-hardy insects are producers of highly effective cryoprotectants, in this study, the hemolymph of Aporia crataegi L. and Upis ceramboides L. from an extremely cold area (Yakutia) was tested as a secondary component of cryoprotective agents (CPA) for cryopreservation (-80 degree C) of human peripheral blood lymphocytes and skin fibroblasts.

Materials and methods: Lymphocytes and skin fibroblasts were treated with various combinations of DMSO and hemolymph extract and step-wise cooled to -80 degree C. Post-cryopreservation cell viability was assessed by vital staining and morphological appearance.

Results: Viability was higher when cells were frozen with a mixture containing DMSO and Upis ceramboides hemolymph compared to the cells frozen in DMSO, while cells frozen with DMSO and Aporia crataegi hemolymph did not survive. The fact that hemolymph of not every cold-resistant insect can be used as a secondary agent along with DMSO indicates that only a unique combination of hemolymph components and its compatibility with cells might result in a positive effect.

Conclusion: Although the use of insect hemolymph as a complementary agent in applied cryopreservation is a problem in terms of practical application, such studies could initiate new trends in the search for the most successful hemolymph-like cryoprotectant systems. https://doi.org/10.54680/fr24210110712.

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测试北极昆虫血淋巴作为应用冷冻保存的辅助剂。
背景:极寒地区昆虫的耐寒性是基于自然低温保护的原理,这与低温保护剂提供的生理机制有关:由于北极耐寒昆虫是高效低温保护剂的生产者,本研究将极寒地区(雅库特)的 Aporia crataegi L. 和 Upis ceramboides L. 的血淋巴作为低温保护剂(CPA)的次要成分进行测试,以低温保存(-80 摄氏度)人类外周血淋巴细胞和皮肤成纤维细胞:用二甲基亚砜和血淋巴提取物的不同组合处理淋巴细胞和皮肤成纤维细胞,然后逐级冷却至零下 80 摄氏度:结果:与用二甲基亚砜冷冻的细胞相比,用含有二甲基亚砜和Upis ceramboides血淋巴的混合物冷冻的细胞存活率更高,而用二甲基亚砜和Aporia crataegi血淋巴冷冻的细胞则无法存活。并非所有耐寒昆虫的血淋巴都能与二甲基亚砜一起用作辅助制剂,这表明只有血淋巴成分的独特组合及其与细胞的相容性才可能产生积极的效果:尽管将昆虫血淋巴用作应用低温保存的辅助剂在实际应用方面存在问题,但此类研究可能会在寻找最成功的血淋巴低温保护剂系统方面引发新的趋势。https://doi.org/10.54680/fr24210110712。
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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