Antibiotic-Resistance Profiles and Genetic Diversity of Shigella Isolates in China: Implications for Control Strategies.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Foodborne pathogens and disease Pub Date : 2024-06-01 Epub Date: 2024-04-01 DOI:10.1089/fpd.2023.0138
Feng Tang, Changzhen Li, Ran Li, Lei Xi, Fang Wang, Juling Tian, Wanjun Luo
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Abstract

The urgent need for comprehensive and systematic analyses of Shigella as the key pathogen led us to meticulously explore the epidemiology and molecular attributes of Shigella isolates. Accordingly, we procured 24 isolates (10 from Xinjiang and 14 from Wuhan, China) and performed serotype identification and antimicrobial susceptibility testing. Resistance gene detection and homology analysis by polymerase chain reaction and pulsed-field gel electrophoresis (PFGE), respectively, were performed for genetic diversity analysis. All isolates were identified as Shigella flexneri, with 70% (35.4-91.9%) and 30% (8.1-64.6%) of the Xinjiang isolates and 85.7% (56.2-97.5%) and 14.3% (2/14, 2.5-43.9%) of the Wuhan isolates belonging to serotype 2a and serotype 2b, respectively. All isolates displayed resistance to at least two antibiotics and complete resistance to ampicillin. Multidrug resistance (MDR) was recorded in 70.8% (48.8-86.6%) of isolates, with Xinjiang isolates exhibiting relatively higher resistance to ampicillin-sulbactam, piperacillin, ceftriaxone, and aztreonam. Conversely, Wuhan isolates displayed higher MDR and resistance to tetracycline, ciprofloxacin, levofloxacin, and cefepime relative to Xinjiang isolates. Molecular scrutiny of antibiotic-resistance determinants revealed that blaTEM was the main mechanism of ampicillin resistance, blaCTX-M was the main gene for resistance to third- and fourth-generation cephalosporins, and tetB was the predominant gene associated with tetracycline resistance. Four Xinjiang and seven Wuhan isolates shared T1-clone types (>85%), and two Xinjiang and one Wuhan isolates were derived from the T6 clone with a high similarity of 87%. Six PFGE patterns (T1, T2, T5, T6-3, T8, and T10) of S. flexneri were associated with MDR. Thus, there is a critical need for robust surveillance and control strategies in managing Shigella infections, along with the development of targeted interventions and antimicrobial stewardship programs tailored to the distinct characteristics of Shigella isolates in different regions of China.

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中国志贺氏菌株的抗生素耐药性特征和遗传多样性:对控制策略的影响》。
由于迫切需要对志贺氏菌这一关键病原体进行全面系统的分析,我们对志贺氏菌分离物的流行病学和分子特征进行了细致的研究。因此,我们获得了 24 个分离株(10 个来自新疆,14 个来自中国武汉),并进行了血清型鉴定和抗菌药敏感性测试。为了进行遗传多样性分析,我们分别通过聚合酶链式反应和脉冲场凝胶电泳(PFGE)进行了耐药基因检测和同源性分析。所有分离物均被鉴定为柔性志贺氏菌,其中新疆分离物中70%(35.4-91.9%)和30%(8.1-64.6%)属于血清2a型,武汉分离物中85.7%(56.2-97.5%)和14.3%(2/14,2.5-43.9%)属于血清2b型。所有分离株都对至少两种抗生素产生耐药性,并对氨苄西林产生完全耐药性。70.8%(48.8%-86.6%)的分离株对多种药物产生耐药性,其中新疆分离株对氨苄西林-舒巴坦、哌拉西林、头孢曲松和阿曲南有较高的耐药性。相反,与新疆分离物相比,武汉分离物对四环素、环丙沙星、左氧氟沙星和头孢吡肟的MDR和耐药性较高。抗生素耐药性决定基因的分子检测显示,blaTEM 是氨苄西林耐药性的主要机制,blaCTX-M 是第三代和第四代头孢菌素耐药性的主要基因,而 tetB 是与四环素耐药性相关的主要基因。4 个新疆分离株和 7 个武汉分离株共享 T1 克隆类型(>85%),2 个新疆分离株和 1 个武汉分离株来自 T6 克隆,相似度高达 87%。flexneri 的六种 PFGE 模式(T1、T2、T5、T6-3、T8 和 T10)与 MDR 相关。因此,在管理志贺氏菌感染时,亟需采取强有力的监测和控制策略,并根据中国不同地区志贺氏菌分离物的不同特点制定有针对性的干预措施和抗菌药物管理计划。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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