PDX1, a transcription factor essential for organ differentiation, regulates SERCA-dependent Ca2+ homeostasis in sensory neurons

IF 4.3 2区 生物学 Q2 CELL BIOLOGY Cell calcium Pub Date : 2024-04-02 DOI:10.1016/j.ceca.2024.102884
Jami L. Saloman , Ariel Y. Epouhe , Catherine F. Ruff , Kathryn M. Albers
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Abstract

Pancreatic and duodenal homeobox 1 (PDX1) is a transcription factor required for the development and differentiation of the pancreas. Previous studies indicated that PDX1 expression was restricted to the gastrointestinal tract. Using a cre-dependent reporter, we observed PDX1-dependent expression of tdtomato (PDX1-tom) in a subpopulation of sensory nerves. Many of these PDX1-tom afferents expressed the neurofilament 200 protein and projected to the skin. Tdtomato-labeled terminals were associated with hair follicles in the form of longitudinal and circumferential lanceolate endings suggesting a role in tactile and proprioceptive perception. To begin to examine the functional significance of PDX1 in afferents, we used Fura-2 imaging to examine calcium (Ca2+) handling under naïve and nerve injury conditions. Neuropathic injury is associated with increased intracellular Ca2+ signaling that in part results from dysregulation of the sarco/endoplasmic reticulum calcium transport ATPase (SERCA). Here we demonstrate that under naïve conditions, PDX1 regulates expression of the SERCA2B isoform in sensory neurons. In response to infraorbital nerve injury, a significant reduction of PDX1 and SERCA2B expression and dysregulation of Ca2+ handling occurs in PDX1-tom trigeminal ganglia neurons. The identification of PDX1 expression in the somatosensory system and its regulation of SERCA2B and Ca2+ handling provide a new mechanism to explain pathological changes in primary afferents that may contribute to pain associated with nerve injury.

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器官分化所必需的转录因子 PDX1 可调节感觉神经元中依赖 SERCA 的 Ca2+ 稳态
胰腺和十二指肠同源框 1(PDX1)是胰腺发育和分化所需的转录因子。以前的研究表明,PDX1 的表达仅限于胃肠道。利用cre依赖性报告基因,我们在感觉神经亚群中观察到了tdtomato(PDX1-tom)的PDX1依赖性表达。其中许多 PDX1-tom 传入神经表达神经丝蛋白 200,并投射到皮肤。Tdtomato标记的末梢以纵向和环向披针形末梢的形式与毛囊相关,这表明它们在触觉和本体感觉中发挥作用。为了开始研究 PDX1 在传入神经中的功能意义,我们使用 Fura-2 成像技术研究了神经损伤条件下的钙离子(Ca2+)处理。神经损伤与细胞内 Ca2+ 信号的增加有关,而 Ca2+ 信号的增加部分源于肌浆/内质网钙离子转运 ATP 酶(SERCA)的失调。我们在此证明,在原始条件下,PDX1 可调节感觉神经元中 SERCA2B 同工酶的表达。在眶下神经损伤后,PDX1 和 SERCA2B 的表达显著减少,PDX1-tom 三叉神经节神经元的 Ca2+ 处理失调。PDX1在躯体感觉系统中的表达及其对SERCA2B和Ca2+处理的调控为解释初级传入神经的病理变化提供了一种新的机制,这种病理变化可能导致与神经损伤相关的疼痛。
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来源期刊
Cell calcium
Cell calcium 生物-细胞生物学
CiteScore
8.70
自引率
5.00%
发文量
115
审稿时长
35 days
期刊介绍: Cell Calcium covers the field of calcium metabolism and signalling in living systems, from aspects including inorganic chemistry, physiology, molecular biology and pathology. Topic themes include: Roles of calcium in regulating cellular events such as apoptosis, necrosis and organelle remodelling Influence of calcium regulation in affecting health and disease outcomes
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