Exosomal LINC00853 promotes progression of gastric cancer via the MAP17/PDZK1/AKT signaling pathway

IF 5.9 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Non-coding RNA Research Pub Date : 2024-03-30 DOI:10.1016/j.ncrna.2024.03.011
Jung-ho Yoon , Hyo Joo Byun , Seo Yeon Kim, Da Hyun Jung, Sang Kil Lee
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Abstract

Although rare, there is ongoing research into biomarkers that predict the onset and recurrence of gastric cancer, particularly focusing on substances found in exosomes. Long non-coding RNAs (lncRNAs) have garnered attention for their potential in diagnosing gastric cancer.

This study investigates the role of lncRNAs in gastric cancer, focusing on their presence in exosomes as potential biomarkers for the disease's onset and recurrence. We utilized the ArrayStar Human LncRNA array 2.0 to analyze lncRNA expression in tissues from early-stage gastric cancer patients. Our analysis highlighted LINC00853, which was significantly upregulated in cancer tissues and implicated in promoting epithelial-mesenchymal transition via the MAP17/PDZK1/AKT pathway. Functional studies on AGS and MKN74 gastric cancer cell lines demonstrated that LINC00853 facilitates cell proliferation, invasion, and migration. Additionally, RNA immunoprecipitation and electrophoretic mobility shift assays confirmed LINC00853 interaction with MAP17. Importantly, LINC00853 was also detected in exosomes from both patient samples and cell lines, and its downregulation led to decreased tumorigenicity in AGS cells. These findings suggest that both cellular and exosomal LINC00853 contribute to gastric cancer pathogenesis and may serve as valuable biomarkers for the disease.

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外泌体LINC00853通过MAP17/PDZK1/AKT信号通路促进胃癌进展
预测胃癌发病和复发的生物标志物虽然罕见,但人们一直在对其进行研究,尤其是对外泌体中发现的物质进行研究。本研究调查了lncRNAs在胃癌中的作用,重点研究了它们作为胃癌发病和复发的潜在生物标记物在外泌体中的存在。我们利用 ArrayStar Human LncRNA array 2.0 分析了早期胃癌患者组织中的 lncRNA 表达。我们的分析强调了 LINC00853,它在癌症组织中明显上调,并与通过 MAP17/PDZK1/AKT 通路促进上皮-间质转化有关。对 AGS 和 MKN74 胃癌细胞系进行的功能研究表明,LINC00853 可促进细胞增殖、侵袭和迁移。此外,RNA免疫沉淀和电泳迁移实验证实了 LINC00853 与 MAP17 的相互作用。重要的是,在患者样本和细胞系的外泌体中也检测到了LINC00853,而下调LINC00853可降低AGS细胞的致瘤性。这些研究结果表明,细胞和外泌体中的LINC00853都有助于胃癌的发病机制,并可作为该疾病有价值的生物标记物。
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来源期刊
Non-coding RNA Research
Non-coding RNA Research Medicine-Biochemistry (medical)
CiteScore
7.70
自引率
6.00%
发文量
39
审稿时长
49 days
期刊介绍: Non-coding RNA Research aims to publish high quality research and review articles on the mechanistic role of non-coding RNAs in all human diseases. This interdisciplinary journal will welcome research dealing with all aspects of non-coding RNAs-their biogenesis, regulation and role in disease progression. The focus of this journal will be to publish translational studies as well as well-designed basic studies with translational and clinical implications. The non-coding RNAs of particular interest will be microRNAs (miRNAs), small interfering RNAs (siRNAs), small nucleolar RNAs (snoRNAs), U-RNAs/small nuclear RNAs (snRNAs), exosomal/extracellular RNAs (exRNAs), Piwi-interacting RNAs (piRNAs) and long non-coding RNAs. Topics of interest will include, but not limited to: -Regulation of non-coding RNAs -Targets and regulatory functions of non-coding RNAs -Epigenetics and non-coding RNAs -Biological functions of non-coding RNAs -Non-coding RNAs as biomarkers -Non-coding RNA-based therapeutics -Prognostic value of non-coding RNAs -Pharmacological studies involving non-coding RNAs -Population based and epidemiological studies -Gene expression / proteomics / computational / pathway analysis-based studies on non-coding RNAs with functional validation -Novel strategies to manipulate non-coding RNAs expression and function -Clinical studies on evaluation of non-coding RNAs The journal will strive to disseminate cutting edge research, showcasing the ever-evolving importance of non-coding RNAs in modern day research and medicine.
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