Validation and optimization of the loop-mediated isothermal amplification (LAMP) technique for rapid detection of wheat stripe mosaic virus, a wheat-infecting pathogen

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology Journal of Genetic Engineering and Biotechnology Pub Date : 2024-04-04 DOI:10.1016/j.jgeb.2024.100373

Anderson Varela de Andrade, Fernando Sartori Pereira, Fabio Nascimento da Silva, Gustavo Felippe da Silva, Maria de Lourdes Borba Magalhães

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Abstract

Background

Wheat stripe mosaic virus (WhSMV) is a significant wheat pathogen that causes substantial yield losses in Brazil and other countries. Although several detection methods are available, reliable and efficient tools for on-site WhSMV detection are currently lacking. In this study, a Loop-Mediated Isothermal Amplification (LAMP) method was developed for rapid and reliable field detection of WhSMV. We designed WhSMV-specific primers for the LAMP assay and optimized reaction conditions for increased sensitivity and specificity using infected plant samples.

Results

We have developed a diagnostic method utilizing the Loop-Mediated Isothermal Amplification (LAMP) technique capable of rapidly and reliably detecting WhSMV. The LAMP assay has been optimized to enhance sensitivity, specificity, and cost-effectiveness.

Conclusion

The LAMP assay described here represents a valuable tool for early WhSMV detection, serving to mitigate the adverse economic and social impacts of this viral pathogen. By enabling swift and accurate identification, this assay can significantly improve the sustainability of cereal production systems, safeguarding crop yields against the detrimental effects of WhSMV.

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验证和优化用于快速检测小麦感染病原体--小麦条纹花叶病毒的环介导等温扩增（LAMP）技术

背景小麦条纹花叶病毒（WhSMV）是一种重要的小麦病原体，在巴西和其他国家造成了巨大的产量损失。虽然已有多种检测方法，但目前仍缺乏可靠、高效的现场检测 WhSMV 的工具。本研究开发了一种环路介导等温扩增（LAMP）方法，用于快速可靠地现场检测 WhSMV。我们为 LAMP 检测设计了 WhSMV 特异性引物，并优化了反应条件，以提高使用受感染植物样本的灵敏度和特异性。结论本文所述的 LAMP 检测法是早期检测 WhSMV 的重要工具，有助于减轻这种病毒病原体对经济和社会的不利影响。通过快速准确的鉴定，该检测方法可以显著提高谷物生产系统的可持续性，保护作物产量免受 WhSMV 的不利影响。

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来源期刊

Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

5.70

自引率

5.70%

发文量

159

审稿时长

16 weeks

期刊介绍： Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts