In silico identification and expression analysis of glutathione S-transferase in Tenebrio molitor

IF 1.2 4区 农林科学 Q3 ENTOMOLOGY Entomological Research Pub Date : 2024-04-05 DOI:10.1111/1748-5967.12720
Ho Am Jang, Seo Jin Lee, Sung Min Ku, Jae Hui Kim, Dong Woo Kang, So Yeon Choi, Sang Mok Jung, Jongdae Lee, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo
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Abstract

Selective herbicides are used to control undesirable vegetation or weeds in fields without harming crops. Herbicide use for weed management can directly impact the densities of insect pests in agricultural communities as a result of insect mortality during and immediately after application. In insects, the glutathione S-transferase (GST) enzyme is involved in both the detoxification process and the defense of cellular membranes against oxidative damage. In this study, two TmGSTs (TmGST-iso1 and TmGST-iso2) were identified and characterized to elucidate the GST family in Tenebrio molitor. Among the developmental stages of T. molitor, eggs had the highest expression levels of TmGST-iso1. TmGST-iso2 expression was highest in the pre-pupal stage. TmGST-iso1 expression was high in the guts of early and late larvae, whereas TmGST-iso2 expression was not observed in early larvae. Adults, both male and female, had the highest levels of TmGST-iso1 mRNA expression in the gut and reproductive organs, and the highest levels of TmGST-iso2 expression in the reproductive organs. Quantitative polymerase chain reaction was used to investigate the impact of treatment with butachlor on the mRNA expression of TmGST-iso1 and TmGST-iso2 in larvae. TmGST-iso1 expression increased in the butachlor-treated group after 3 and 24 h, whereas TmGST-iso2 expression peaked at 24 h after treatment. This study provides vital information about the detoxifying activities of T. molitor.

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谷胱甘肽 S 转移酶的硅学鉴定和表达分析
选择性除草剂用于控制田间的不良植被或杂草,而不伤害作物。使用除草剂治理杂草会直接影响农业区害虫的密度,因为施药期间和施药后昆虫会立即死亡。在昆虫体内,谷胱甘肽 S-转移酶(GST)既参与解毒过程,又保护细胞膜免受氧化损伤。本研究发现了两种 TmGST(TmGST-iso1 和 TmGST-iso2),并对其进行了鉴定,以阐明褐飞虱体内的 GST 家族。在褐天牛的各个发育阶段中,卵的 TmGST-iso1 表达水平最高。TmGST-iso2在蛹前期的表达量最高。在早期和晚期幼虫的内脏中,TmGST-iso1 的表达量较高,而在早期幼虫中则未观察到 TmGST-iso2 的表达。成虫(包括雌雄)肠道和生殖器官中的 TmGST-iso1 mRNA 表达水平最高,生殖器官中的 TmGST-iso2 表达水平最高。利用定量聚合酶链反应研究了丁草胺处理对幼虫体内 TmGST-iso1 和 TmGST-iso2 mRNA 表达的影响。结果表明,丁草胺处理组的TmGST-iso1表达量在3小时和24小时后有所增加,而TmGST-iso2的表达量在处理后24小时达到峰值。这项研究为了解褐飞虱的解毒活性提供了重要信息。
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来源期刊
CiteScore
2.50
自引率
7.70%
发文量
64
期刊介绍: Entomological Research is the successor of the Korean Journal of Entomology. Published by the Entomological Society of Korea (ESK) since 1970, it is the official English language journal of ESK, and publishes original research articles dealing with any aspect of entomology. Papers in any of the following fields will be considered: -systematics- ecology- physiology- biochemistry- pest control- embryology- genetics- cell and molecular biology- medical entomology- apiculture and sericulture. The Journal publishes research papers and invited reviews.
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