Yong Ye, Guang Xia, Min Chen, Jifu Jin, Linxiang Lu, Xin Wang
{"title":"MiR-92a Promotes Apoptosis in Rats with Myocardial Ischemia-Reperfusion Injury via Regulating Wnt/β-Catenin Pathway","authors":"Yong Ye, Guang Xia, Min Chen, Jifu Jin, Linxiang Lu, Xin Wang","doi":"10.1166/jbn.2024.3818","DOIUrl":null,"url":null,"abstract":"In this study, the impact of micro ribonucleic acid (miR)-92a on rats with myocardial ischemia-reperfusion injury was investigated, with a focus on its regulation of the Wnt/β-catenin pathway. A total of 36 Sprague Dawley rats were divided into three groups: a sham operation\n group, a model group, and a miR-92a antagomir group. The sham group underwent thoracotomy without injury, while the model and miR-92a antagomir groups were subjected to myocardial ischemiareperfusion injury and treated with saline and miR-92a antagomir, respectively. Results showed that the\n myocardial infarction area was significantly reduced in the miR-92a antagomir group compared to the model group. Histological analysis revealed improved myocardial tissue structure in the miR-92a antagomir group. Western blotting demonstrated elevated levels of p-GSK-3β and β-catenin\n in both the model and miR-92a antagomir groups, with a notable decrease in the miR-92a antagomir group compared to the model group. Additionally, miR-92a expression was higher in both the model and miR-92a antagomir groups compared to the sham group. Lastly, apoptosis rates were increased\n in both the model and miR-92a antagomir groups, but significantly reduced in the miR-92a antagomir group compared to the model group. Overall, these findings suggest that miR-92a exacerbates apoptosis in rats with myocardial ischemia-reperfusion injury by up-regulating the Wnt/β-catenin\n signaling pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2024.3818","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, the impact of micro ribonucleic acid (miR)-92a on rats with myocardial ischemia-reperfusion injury was investigated, with a focus on its regulation of the Wnt/β-catenin pathway. A total of 36 Sprague Dawley rats were divided into three groups: a sham operation
group, a model group, and a miR-92a antagomir group. The sham group underwent thoracotomy without injury, while the model and miR-92a antagomir groups were subjected to myocardial ischemiareperfusion injury and treated with saline and miR-92a antagomir, respectively. Results showed that the
myocardial infarction area was significantly reduced in the miR-92a antagomir group compared to the model group. Histological analysis revealed improved myocardial tissue structure in the miR-92a antagomir group. Western blotting demonstrated elevated levels of p-GSK-3β and β-catenin
in both the model and miR-92a antagomir groups, with a notable decrease in the miR-92a antagomir group compared to the model group. Additionally, miR-92a expression was higher in both the model and miR-92a antagomir groups compared to the sham group. Lastly, apoptosis rates were increased
in both the model and miR-92a antagomir groups, but significantly reduced in the miR-92a antagomir group compared to the model group. Overall, these findings suggest that miR-92a exacerbates apoptosis in rats with myocardial ischemia-reperfusion injury by up-regulating the Wnt/β-catenin
signaling pathway.