Yaya Zhou, Xinliang He, Jian Tang, Dongmei Zhang, Yao Liu, Y. Xue, Nanchuan Jiang, Jianchu Zhang, Xiaorong Wang
{"title":"Total sputum nitrate/nitrite is associated with exacerbations andPseudomonas aeruginosacolonization in bronchiectasis","authors":"Yaya Zhou, Xinliang He, Jian Tang, Dongmei Zhang, Yao Liu, Y. Xue, Nanchuan Jiang, Jianchu Zhang, Xiaorong Wang","doi":"10.1183/23120541.01045-2023","DOIUrl":null,"url":null,"abstract":"Sputum nitrate/nitrite, which is the main component of reactive nitrogen species (RNS), is a potential biomarker of disease severity and progression in bronchiectasis. This study aimed to determine the association between nitrate/nitrite and exacerbations, airway microbiota in bronchiectasis.We measured total nitrate/nitrite concentration in sputum samples collected from 85 patients with stable bronchiectasis, performed 16 s rRNA sequencing of sputum samples and predicted the denitrification ability of airway microbiota through PICRUSt. Relationships between sputum total nitrate/nitrite and disease severity, exacerbations and airway microbiota were examined.Higher total sputum nitrate/nitrite was associated with more severe bronchiectasis defined by E-FACED (p= 0.003) or BSI (p=0.006) and more exacerbations in prior 12 month (p=0.005). Moreover, total sputum nitrate/nitrite was also significantly higher in patients with worse cough score (p=0.03), worse sputum purulent score (p=0.01), and worse MRC score (p=0.02). In addition, the total sputum nitrate/nitrite ofP. aeruginosacolonization (PA) group was higher than that of non-P. aeruginosacolonization (NPA) group (p=0.04), meanwhile, relative abundance ofP. aeruginosawas positively correlated with the total nitrate/nitrite (r=0.337, p=0.002). Simultaneously, denitrification module (M00529) was also significantly enriched in PA group compared to NPA group through PICRUSt analyses. Using receiver-operating characteristic analysis, total nitrate/nitrite was associated with exacerbations during one year follow-up (AUC=0.741, p=0.014).Sputum nitrate/nitrite is a biomarker of disease severity and associated withP. aeruginosacolonization in bronchiectasis.","PeriodicalId":11739,"journal":{"name":"ERJ Open Research","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ERJ Open Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1183/23120541.01045-2023","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}
引用次数: 0
Abstract
Sputum nitrate/nitrite, which is the main component of reactive nitrogen species (RNS), is a potential biomarker of disease severity and progression in bronchiectasis. This study aimed to determine the association between nitrate/nitrite and exacerbations, airway microbiota in bronchiectasis.We measured total nitrate/nitrite concentration in sputum samples collected from 85 patients with stable bronchiectasis, performed 16 s rRNA sequencing of sputum samples and predicted the denitrification ability of airway microbiota through PICRUSt. Relationships between sputum total nitrate/nitrite and disease severity, exacerbations and airway microbiota were examined.Higher total sputum nitrate/nitrite was associated with more severe bronchiectasis defined by E-FACED (p= 0.003) or BSI (p=0.006) and more exacerbations in prior 12 month (p=0.005). Moreover, total sputum nitrate/nitrite was also significantly higher in patients with worse cough score (p=0.03), worse sputum purulent score (p=0.01), and worse MRC score (p=0.02). In addition, the total sputum nitrate/nitrite ofP. aeruginosacolonization (PA) group was higher than that of non-P. aeruginosacolonization (NPA) group (p=0.04), meanwhile, relative abundance ofP. aeruginosawas positively correlated with the total nitrate/nitrite (r=0.337, p=0.002). Simultaneously, denitrification module (M00529) was also significantly enriched in PA group compared to NPA group through PICRUSt analyses. Using receiver-operating characteristic analysis, total nitrate/nitrite was associated with exacerbations during one year follow-up (AUC=0.741, p=0.014).Sputum nitrate/nitrite is a biomarker of disease severity and associated withP. aeruginosacolonization in bronchiectasis.
期刊介绍:
ERJ Open Research is a fully open access original research journal, published online by the European Respiratory Society. The journal aims to publish high-quality work in all fields of respiratory science and medicine, covering basic science, clinical translational science and clinical medicine. The journal was created to help fulfil the ERS objective to disseminate scientific and educational material to its members and to the medical community, but also to provide researchers with an affordable open access specialty journal in which to publish their work.