Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran

IF 0.9 4区医学 Q4 PARASITOLOGY Iranian Journal of Parasitology Pub Date : 2024-03-17 DOI:10.18502/ijpa.v19i1.15216

Elaheh Hezardastan, Ali - Fattahi Bafghi, G. Eslami, Mahmoud Vakili

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Abstract

Background: We aimed to investigate the molecular genotyping of Giardia duodenalis in Humans in the Yazd County, Central, Iran. Methods: Total of 35 fecal samples were collected from patients referred to Yazd Central Laboratory, Yazd, Iran from February to July 2022. All the samples were included in this study after microscopic observation of G. duodenalis. DNA samples were extracted using related kit and were analyzed by Nano Drop. The molecular assessment was carried out using semi-nested PCR using the target gene of gdh. All amplified samples were sequenced using Sanger method. BLAST analyzed the sequences for assemblage identification. Results: Out of 35 samples, 24 (68.57%) and 11 (31.43%) were male and female, respectively. All included samples were amplified using the specific gdh primer pair. The molecular analysis showed 17 isolates (48.57%) as assemblage BIV, 8 isolates (22.86%) as assemblage BIII, 6 isolates (17.14%) as assemblage AII and 4 isolates (11.43%) as assemblage AIII (P<0.05). Conclusion: Assemblages A and B are the most prevalent in Central Iran. The molecular identification of G. duodenalis isolates from animals and implementing control programs

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伊朗中部亚兹德县人类十二指肠贾第虫的分子基因分型

背景：我们旨在研究伊朗中部亚兹德县人类中十二指肠贾第虫的分子基因分型。研究方法从 2022 年 2 月到 7 月，我们从转诊到伊朗亚兹德中心实验室的患者身上共采集了 35 份粪便样本。所有样本均在显微镜下观察到十二指肠球菌（G. duodenalis）后纳入本研究。使用相关试剂盒提取 DNA 样本，并用 Nano Drop 进行分析。分子评估采用半嵌套 PCR 方法，使用 gdh 目标基因。使用 Sanger 方法对所有扩增样本进行测序。BLAST 对序列进行了分析，以确定组合。结果：在 35 个样本中，男性和女性样本分别为 24 个（68.57%）和 11 个（31.43%）。所有样本均使用特定的 gdh 引物对进行扩增。分子分析表明，17 个分离物（48.57%）属于 BIV 组合，8 个分离物（22.86%）属于 BIII 组合，6 个分离物（17.14%）属于 AII 组合，4 个分离物（11.43%）属于 AIII 组合（P<0.05）。结论A 和 B 组合在伊朗中部最为普遍。从动物中分离出的十二指肠球菌的分子鉴定与控制计划的实施

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来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.