HPTLC Analysis of Aragwadhadi Kashaya and its Ingredients

Sanitha U M, R.Rajam
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Abstract

Decoctions, are basically the extraction of medicines into water by means of boiling. In Ayurveda it is known by the name ‘kwatha’, ‘kashaya’,’sritha’ and ‘niryooha’. The term kwatha is derived from word ‘kwathana’ which means boiling. Aragwadhadhi kashaya is mentioned in Sharngadhara Samhitha, ingredients of which are Aragwadha, Kanamoola, Tiktha, Mustha and Abhaya. All the ingredients were checked for its identity, purity and strength and only those satisfying the API parameters were taken for the study. Kashaya was prepared as per classical method mentioned in Sharngadhara Samhitha. The prepared kashaya was lyophilised and further this extract was used for analysis. The extract of decoction and extracts of individual ingredients were analysed HPTLC was done with two different concentrations of kashaya extract and extract of five individual ingredients in a single Merck, HPTLC Silica gel 60 F254 plate. Quantification was not done in this study, but the results provided valuable information on the presence of various compounds in the decoction. Further research could focus on quantifying these compounds to better understand their concentrations and potential effects. Rf values obtained from two different concentrations of kashaya extract were not exactly similar. There were very few identical Rf values in individual ingredients and kashaya extract.
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Aragwadhadi Kashaya 及其成分的 HPTLC 分析
煎煮基本上是通过煮沸的方式将药物提取到水中。在阿育吠陀中,它被称为 "kwatha"、"kashaya"、"sritha "和 "niryooha"。kwatha 一词源于 "kwathana",意思是沸腾。Aragwadhadhi kashaya 在《Sharngadhara Samhitha》中有所提及,其成分包括 Aragwadha、Kanamoola、Tiktha、Mustha 和 Abhaya。所有成分都经过了身份、纯度和强度检查,只有符合 API 参数的成分才被用于研究。卡沙亚是按照 Sharngadhara Samhitha 中提到的经典方法制备的。制备好的 Kashaya 被冻干,然后将提取物用于分析。在一个默克 HPTLC 硅胶 60 F254 板上,用两种不同浓度的卡沙亚提取物和五种不同成分的提取物进行 HPTLC 分析。这项研究没有进行定量分析,但结果提供了煎药中各种化合物的宝贵信息。进一步的研究可以侧重于对这些化合物进行定量,以便更好地了解它们的浓度和潜在作用。从两种不同浓度的卡沙亚提取物中获得的 Rf 值并不完全相似。个别成分和卡沙亚提取物中很少有相同的 Rf 值。
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