Phytoconstituents, antioxidant, and cholinesterase inhibitory activities of the leaves and stem extracts of Artocarpus sericicarpus

Pharmacia Pub Date : 2024-02-19 DOI:10.3897/pharmacia.71.e112499

Suciati Suciati, E. R. Laili, Hamizah Haula, L. Tumewu, N. Nuengchamnong, N. Suphrom, A. Widyawaruyanti

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Abstract

The study aimed to investigate the antioxidant and cholinesterase inhibitory activities of the leaves and stems of Artocarpus sericicarpus and to analyse the phenolic compounds in the extracts. The modified Ellman’s method was used to determine the cholinesterase inhibitory activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. The antioxidant properties were evaluated using DPPH and ABTS methods. The total phenolic content (TPC) was measured by spectrometric assay, and compound identification was carried out by LC-MS/MS analysis. The results showed that the leaf and stem extracts of A. sericicarpus exerted significant inhibitory effects against AChE and BChE, as well as antioxidant activities. The stem ethanolic extract exhibited the highest potency against AChE and BChE with IC50 values of 5.81 and 11.46 µg/mL, respectively. The leaf and stem ethanolic extracts gave higher antioxidant activities and TPC compared to the water-based extracts. The LC-MS/MS analysis indicated the presence of phenolic compounds, such as flavones, flavonols, flavanones, prenylated chalcones, and xanthones in the extracts.

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芹菜叶和茎提取物的植物成分、抗氧化性和胆碱酯酶抑制活性

本研究旨在调查芹菜叶和茎的抗氧化和胆碱酯酶抑制活性，并分析提取物中的酚类化合物。采用改进的埃尔曼法测定胆碱酯酶对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制活性。抗氧化性采用 DPPH 和 ABTS 法进行评估。总酚含量（TPC）通过光谱分析法测定，化合物鉴定通过 LC-MS/MS 分析法进行。结果表明，A. sericarpus 的叶和茎提取物对 AChE 和 BChE 有显著的抑制作用，并具有抗氧化活性。茎乙醇提取物对 AChE 和 BChE 的抑制作用最强，IC50 值分别为 5.81 和 11.46 µg/mL。与水基提取物相比，叶和茎乙醇提取物具有更高的抗氧化活性和 TPC。LC-MS/MS 分析表明，提取物中含有酚类化合物，如黄酮、黄酮醇、黄烷酮、前炔基查耳酮和黄酮。

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Pharmacia

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