Understanding the long-lasting associations among humans, neglected dogs, tick-borne diseases, and core blood bacteria-related pathogenic taxa using next-generation sequencing

S. I. BARRAZA-GUERRERO, C. García-De la Peña, C. Meza-Herrera, Q. Siller-Rodríguez, F. Vaca-Paniagua, C. Díaz-Velásquez, A. D. L. Cruz-Montoya, L. M. Valenzuela-Núñez
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Abstract

Background and Aim: Dogs are long-lasting companion animals, and ticks are the most common external parasites in dogs. An increase in the population of neglected domestic dogs has increased the risk of contact with ticks, especially in places where tick-borne diseases (TBDs) are endemic. We aimed to characterize the bacterial blood profiles of people who were either exposed (HE) or not exposed (HC) to tick bites using next-generation sequencing (NGS). Materials and Methods: In the present study, the bacteria observed in the blood of people exposed to tick bites were compared with those in the blood of people not exposed to tick bites in Northern Mexico. Human blood samples (n = 12) were analyzed, DNA was extracted, and the V3–V4 region of the 16S ribosomal RNA gene was amplified. In addition, NGS was performed on a MiSeq platform (Illumina), and the data were analyzed through Quantitative Insights into Microbial Ecology. Results: Differences in beta diversity were significant. In HEs, several potentially pathogenic bacterial taxa were found to be the most abundant: Kocuria ( x = 14.59%), Staphylococcus ( x = 3.05%), and Treponema ( x = 2.93%), in addition to Chlamydia, Clostridium, and Ehrlichia, which are considered TBDs. Conclusion: This study identified important differences in the bacterial composition of the HE and HC groups. In addition to Ehrlichia (a TBD considered a taxon), other bacterial pathogenic taxa, such as Chlamydia, Clostridium, Kocuria, Staphylococcus, and Treponema, were also observed in the tick bite-exposed group. Future studies with larger sample sizes should provide an improved understanding of the human blood microbiome profile by providing additional evidence of tick exposure, associated TBDs, and other pathogenic bacterial taxa. Keywords: Blood, Ehrlichia, microbiome, tick bites, tick-borne diseases, ticks.
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利用新一代测序技术了解人类、被忽视的狗、蜱传疾病和核心血液细菌相关致病类群之间的长期联系
背景和目的:狗是长寿的伴侣动物,而蜱虫是狗最常见的体外寄生虫。被忽视的家犬数量的增加增加了与蜱虫接触的风险,尤其是在蜱传疾病(TBDs)流行的地方。我们的目的是利用新一代测序技术(NGS)分析暴露于蜱虫叮咬(HE)或未暴露于蜱虫叮咬(HC)的人群的细菌血液特征:在本研究中,对墨西哥北部被蜱虫叮咬者血液中的细菌与未被蜱虫叮咬者血液中的细菌进行了比较。对人体血液样本(n = 12)进行分析,提取 DNA 并扩增 16S 核糖体 RNA 基因的 V3-V4 区域。此外,还在 MiSeq 平台(Illumina)上进行了 NGS,并通过微生物生态学定量洞察对数据进行了分析:结果:贝塔多样性差异显著。在 HE 中,几个潜在致病细菌类群的数量最多:除了衣原体、梭状芽孢杆菌和埃希氏菌(被认为是 TBDs)之外,Kocuria ( x = 14.59%)、Staphylococcus ( x = 3.05%)和 Treponema ( x = 2.93%)也是如此:本研究发现了 HE 组和 HC 组细菌组成的重要差异。除埃希氏菌(TBD类群)外,在被蜱叮咬组中还观察到其他细菌致病类群,如衣原体、梭菌、球菌、葡萄球菌和特雷波纳菌。未来样本量更大的研究应能提供更多关于蜱暴露、相关TBDs和其他致病细菌类群的证据,从而加深对人类血液微生物组概况的了解:血液 埃里希氏菌 微生物组 蜱叮咬 蜱传疾病 蜱
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来源期刊
International Journal of One Health
International Journal of One Health Veterinary-Veterinary (all)
CiteScore
2.10
自引率
0.00%
发文量
13
审稿时长
15 weeks
期刊介绍: International Journal of One Health publishes high quality and novelty papers focusing on One Health. Review articles are highly appreciated. All articles published by International Journal of One Health are made freely and permanently accessible online. All articles to International Journal of One Health are posted online immediately as they are ready for publication.
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