Benzydamine hydrochloride ameliorates ethanol-induced inflammation in RAW 264.7 macrophages by stabilizing redox homeostasis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-02-01 DOI:10.4103/apjtb.apjtb_823_23

Tiasha Dasgupta, Venkatraman Manickam

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Abstract

To evaluate the protective effect of benzydamine hydrochloride against ethanol-induced oxidative stress and inflammation in RAW 264.7 macrophages. RAW 264.7 macrophages were treated with ethanol (100 mM) and benzydamine hydrochloride (7.5 μM). The inflammatory status was confirmed by measuring pro-(TNF-α and IL-6) and anti-inflammatory (IL-10) cytokines through ELISA and RT-PCR assays. Reactive oxygen species generation and mitochondrial membrane potential were investigated to study the protective role of benzydamine hydrochloride against ethanol-induced oxidative stress. Apoptosis detection was also investigated using flow cytometry and acridine orange/ethidium bromide staining. Benzydamine hydrochloride significantly decreased the secretion of TNF-α and IL-6, as well as the generation of reactive oxygen species inside the cells, thereby stabilizing the mitochondrial membrane potential and reducing DNA fragmentation. The ethanol-induced cellular necrosis was also reversed by the administration of benzydamine hydrochloride. Benzydamine hydrochloride ameliorates ethanol-induced cell apoptosis and inflammation in RAW macrophages.

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盐酸苄达明通过稳定氧化还原平衡改善乙醇诱导的 RAW 264.7 巨噬细胞炎症反应

评估盐酸苄达明对乙醇诱导的 RAW 264.7 巨噬细胞氧化应激和炎症的保护作用。用乙醇（100 mM）和盐酸苄达明（7.5 μM）处理 RAW 264.7 巨噬细胞。通过酶联免疫吸附和 RT-PCR 法检测促炎细胞因子（TNF-α 和 IL-6）和抗炎细胞因子（IL-10），确认炎症状态。为了研究盐酸苄达明对乙醇诱导的氧化应激的保护作用，还对活性氧生成和线粒体膜电位进行了调查。此外，还使用流式细胞术和吖啶橙/溴化乙锭染色法检测了细胞凋亡。盐酸苄达明明显减少了 TNF-α 和 IL-6 的分泌以及细胞内活性氧的生成，从而稳定了线粒体膜电位并减少了 DNA 断裂。服用盐酸苄达明还能逆转乙醇诱导的细胞坏死。盐酸苄达明可改善乙醇诱导的 RAW 巨噬细胞细胞凋亡和炎症。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.