{"title":"BIOLOGICAL FEATURES OF HEDYSARUM ALPINUM L. CELL CULTUREWHEN GROWN IN VITRO","authors":"P. S. Savin, N. S. Tsybulko, S. B. Myasnikova","doi":"10.29296/25877313-2024-01-08","DOIUrl":null,"url":null,"abstract":"The purpose of the research conducted was to obtain a cell culture of Hedysarum alpicum, as a producer of xanthone mangiferin, by achieving the tasks of obtaining a cell culture of H. alpium growing in deep-cultivation conditions and studying its physiological characteristics. \nMaterial and methods. The object of the study was a cell culture of H. alpine obtained from the cotyledon of the germination, strain H. alpinum (C) 2016 of the collection of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants» and the native plant grown on the pharmacopeial site of the Botanical Garden of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants», harvest of 2013. \nResults. It has been established that growth regulators kinetin and 2.4D stimulate growth processes of H. alpium (C) cell culture 2016 in deep cultivation conditions. The duration of growth is 20–23 days. The maximum increase corresponds to 16 g/l on the 20th day of growth. The maximum consumption of oxygen by the cell culture is 30·104 mol/l·h on the 16th day of growth, when the cells are in the regular growth phase. \nConclusion. As a result of the work carried out, a stable cell strain of H. alpium was obtained, growing on the surface of the agarized nutrient medium, which has the potential for the synthesis of mangiferin and work has been carried out on studying its physiological characteristics.","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":"84 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Problems Of Biological, Medical And Pharmaceutical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29296/25877313-2024-01-08","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The purpose of the research conducted was to obtain a cell culture of Hedysarum alpicum, as a producer of xanthone mangiferin, by achieving the tasks of obtaining a cell culture of H. alpium growing in deep-cultivation conditions and studying its physiological characteristics.
Material and methods. The object of the study was a cell culture of H. alpine obtained from the cotyledon of the germination, strain H. alpinum (C) 2016 of the collection of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants» and the native plant grown on the pharmacopeial site of the Botanical Garden of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants», harvest of 2013.
Results. It has been established that growth regulators kinetin and 2.4D stimulate growth processes of H. alpium (C) cell culture 2016 in deep cultivation conditions. The duration of growth is 20–23 days. The maximum increase corresponds to 16 g/l on the 20th day of growth. The maximum consumption of oxygen by the cell culture is 30·104 mol/l·h on the 16th day of growth, when the cells are in the regular growth phase.
Conclusion. As a result of the work carried out, a stable cell strain of H. alpium was obtained, growing on the surface of the agarized nutrient medium, which has the potential for the synthesis of mangiferin and work has been carried out on studying its physiological characteristics.
本研究的目的是通过获得在深层栽培条件下生长的白花蛇舌草细胞培养物并研究其生理特点,从而获得可生产黄酮芒果苷的白花蛇舌草细胞培养物。材料和方法研究对象为从FSBSI "全俄药用和芳香植物科学研究院"(All-Russian Scientific Research Institute of Medicinal and Aromatic Plants)收藏的2016年高山乌头(H. alpinum (C))菌株发芽子叶中获得的高山乌头细胞培养物,以及2013年收获的生长在FSBSI "全俄药用和芳香植物科学研究院"(All-Russian Scientific Research Institute of Medicinal and Aromatic Plants)植物园药用地的原生植物。研究结果经证实,生长调节剂激肽素和 2.4D 能在深层栽培条件下刺激 H. alpium (C) 细胞培养 2016 的生长过程。生长持续时间为 20-23 天。在生长的第 20 天,最大增幅相当于 16 克/升。细胞培养的最大耗氧量为生长第 16 天的 30-104 mol/l-h,此时细胞处于常规生长阶段。结论通过这项工作,获得了一种稳定的 H. alpium 细胞株,它生长在琼脂营养培养基表面,具有合成芒果苷的潜力,并已着手研究其生理特征。