Screening of Rapeseed Genotypes against Alternaria Leaf Blight Resistance at Nawalpur, Sarlahi

P. Wagle, B. P. Yadav, Santosh Rasaily, Anand Chaudhary, A. Mishra, S. Subedi
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Abstract

: Rapeseed is major oilseed crop of Nepal but its yield is limited due to various factors and one of the major limiting factors is leaf blight disease caused by Alternaria brassicae (Berk.). An experiment was conducted at Oilseed research Program, Nawalpur, Sarlahi, Nepal during two consecutive years viz. 2019 and 2020 to screen the rapeseed genotypes against alternaria leaf blight disease. The research was conducted in augments design with each genotype sown in two rows of 3 m length 30 cm apart and disease was allowed to develop naturally in the field. Four times scoring of disease was done starting from 49 days after sowing at seven days interval. Out of one hundred and ninety genotypes used in evaluation, there were no genotypes that were immune or completely resistant to the disease but thirty one genotypes during 2019 and eight genotypes during 2020 were found to be moderately resistant to the disease. One genotype ICT 2010-7 was found to be moderately susceptible in both the years’ observations. Area under disease progress curve (AUDPC) value for three genotypes (ICT 2001-6, ICT 2001-7, and ICT 2010-9) during 2019 and six genotypes (ICT 2010-7, S R O 2, ICT 2004-1, NGRC 2798, ICT 2004-42, and ICT 2006-3) during 2020 was lower (<400) representing lower disease progress. Hence the genotypes that were moderately resistant as well as have lower AUDPC value can be utilized in future by plant breeders and plant pathologist for development of tolerant varieties as durable resource for disease management.
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在萨尔拉希的纳瓦尔布尔对油菜籽基因型进行叶枯病抗性筛选
:油菜籽是尼泊尔的主要油料作物,但由于各种因素,其产量受到限制,其中一个主要限制因素是由黄铜交替菌(Alternaria brassicae (Berk.))引起的叶枯病。2019 年和 2020 年连续两年在尼泊尔萨尔拉希纳瓦尔布尔的油菜籽研究计划中进行了一项实验,以筛选油菜籽基因型,使其免受交替孢属叶枯病的侵害。研究采用增量设计,每个基因型播种两行,每行 3 米长,间距 30 厘米,让病害在田间自然发展。从播种后 49 天开始进行四次病害评分,每次间隔七天。在用于评估的 190 个基因型中,没有免疫或完全抗病的基因型,但发现 2019 年的 31 个基因型和 2020 年的 8 个基因型具有中度抗病性。有一个基因型 ICT 2010-7 在这两年的观察中都被发现中度易感。2019 年的三个基因型(ICT 2001-6、ICT 2001-7 和 ICT 2010-9)和 2020 年的六个基因型(ICT 2010-7、S R O 2、ICT 2004-1、NGRC 2798、ICT 2004-42 和 ICT 2006-3)的病害进程曲线下面积(AUDPC)值较低(<400),表明病害进程较低。因此,植物育种者和植物病理学家今后可利用抗性适中且 AUDPC 值较低的基因型开发抗病品种,作为病害管理的持久资源。
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