Comparative assessment of cytospin preparation of a few lymphoid organs of pig

Abstract

The study of cell suspension preparation is important for detecting neoplastic conditions of the organs concerned. Cell suspensions were prepared from lymph nodes, Peyer’s patches and spleen. The cell suspensions were centrifuged in Labofuge-400 and cytospots were prepared on micro slides. The cytospots were stained with Giemsa and Leishman’s stain and were compared. The lymph nodes, Peyer’s patches and spleen cytospots were predominantly occupied by lymphocytes which were of different sizes and shapes. Besides, mucus-secreting goblet cells were seen in Peyer’s patch cytospot. In splenic cytospot, other leukocyte varieties were also noticed like the neutrophils and the eosinophils. It was observed that Giemsa staining gave better results in all cases compared to Leishman’s stain in terms of better contrast between cytoplasm and nucleus. Many types of cells with different morphology were seen in all the preparations. However, it was difficult to identify the cells employing normal microscopy and normal Giemsa or Leishman’s staining. The same cytospots may be subjected to immuno-staining under immunocytochemistry to differentiate various types of cells.