Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2024-07-01 DOI:10.1016/j.chmed.2023.10.004
Wenjun Wei , Tao Guo , Wenguang Fan , Mengshan Ji , Yu Fu , Conglong Lian , Suiqing Chen , Wenjing Ma , Wenfang Ma , Shuying Feng
{"title":"Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus","authors":"Wenjun Wei ,&nbsp;Tao Guo ,&nbsp;Wenguang Fan ,&nbsp;Mengshan Ji ,&nbsp;Yu Fu ,&nbsp;Conglong Lian ,&nbsp;Suiqing Chen ,&nbsp;Wenjing Ma ,&nbsp;Wenfang Ma ,&nbsp;Shuying Feng","doi":"10.1016/j.chmed.2023.10.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p><em>Lilium brownii</em> var. <em>viridulum</em> (LB) and <em>L. lancifolium</em> (LL) are the main sources of medicinal lily (<em>Lilii Bulbus</em>, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of <em>Lilii Bulbus</em>, we selected <em>L. davidii</em> var. <em>willmottiae</em> (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL).</p></div><div><h3>Methods</h3><p>Metabolome and transcriptome were carried out to investigate the differences of active components in LD <em>vs</em> LB and LD <em>vs</em> LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCA), and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis.</p></div><div><h3>Results</h3><p>The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD <em>vs</em> LB group and 298 SDMs in LD <em>vs</em> LL group. Compared with LD group, the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCA of transcriptome indicated that there was significant difference in LB <em>vs</em> LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD <em>vs</em> LB group and 18 958 DEGs in LD <em>vs</em> LL group were identified. Notably, CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected, and comparing with LD, <em>CYP724</em>, <em>CYP710A</em>, and <em>CYP734A1</em> in LB and <em>CYP90B</em> in LL were all up-regulated.</p></div><div><h3>Conclusion</h3><p>This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of <em>Lilii Bulbus</em>, which can provide new insights for <em>Lilii Bulbus</em> used in the research and development of classic famous formula.</p></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1674638424000078/pdfft?md5=36e4859dc837025c25ed32688316af0b&pid=1-s2.0-S1674638424000078-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Herbal Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1674638424000078","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0

Abstract

Objective

Lilium brownii var. viridulum (LB) and L. lancifolium (LL) are the main sources of medicinal lily (Lilii Bulbus, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of Lilii Bulbus, we selected L. davidii var. willmottiae (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL).

Methods

Metabolome and transcriptome were carried out to investigate the differences of active components in LD vs LB and LD vs LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCA), and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis.

Results

The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD vs LB group and 298 SDMs in LD vs LL group. Compared with LD group, the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCA of transcriptome indicated that there was significant difference in LB vs LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD vs LB group and 18 958 DEGs in LD vs LL group were identified. Notably, CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected, and comparing with LD, CYP724, CYP710A, and CYP734A1 in LB and CYP90B in LL were all up-regulated.

Conclusion

This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of Lilii Bulbus, which can provide new insights for Lilii Bulbus used in the research and development of classic famous formula.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
代谢组和转录组的整合分析为了解莉莉牛的功能成分提供了新的视角
目的 棕百合(Lilium brownii var.viridulum,LB)和白百合(Lilium brownii var.viridulum,LL)是中国药用百合的主要来源。然而,这两种百合中具有治疗功效的功能成分尚不明确。方法通过代谢组和转录组研究LD与LB、LD与LL中有效成分的差异。采用主成分分析(PCA)、层次聚类分析(HCA)等多种分析方法对代谢组和转录组数据进行分析。结果 代谢组的 PCA 和 HCA 分析表明,与 LD 相比,LL 和 LB 中的代谢物明显分离且差异很大。在 LD 组和 LB 组中,有 318 个代谢物存在明显差异(SDMs),在 LD 组和 LL 组中,有 298 个代谢物存在明显差异(SDMs)。与 LD 组相比,LB 组和 LL 组的甾体皂甙和甾体生物碱都有明显的上调,尤其是在 LB 组。转录组的 HCA 显示,LB 组与 LD 组之间存在显著差异,而 LL 组与 LD 组之间的差异略有不同。此外,在 LD 组与 LB 组中发现了 47 540 个 DEGs,在 LD 组与 LL 组中发现了 18 958 个 DEGs。与 LD 组相比,LB 组的 CYP724、CYP710A 和 CYP734A1 以及 LL 组的 CYP90B 均上调。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
期刊最新文献
HPLC combined with chemometrics for quality control of Huamoyan Granules or Capsules Constituents from leaves of Macaranga hemsleyana Chemical constituents, pharmacological activities and quality evaluation methods of genus Hippocampus: A comprehensive review Ethnopharmacology, chemical composition and functions of Cymbopogon citratus Bioactive components, pharmacological properties and underlying mechanism of Ganoderma lucidum spore oil: A review
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1