Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2024-07-01 DOI:10.1016/j.chmed.2023.10.004
Wenjun Wei , Tao Guo , Wenguang Fan , Mengshan Ji , Yu Fu , Conglong Lian , Suiqing Chen , Wenjing Ma , Wenfang Ma , Shuying Feng
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Abstract

Objective

Lilium brownii var. viridulum (LB) and L. lancifolium (LL) are the main sources of medicinal lily (Lilii Bulbus, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of Lilii Bulbus, we selected L. davidii var. willmottiae (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL).

Methods

Metabolome and transcriptome were carried out to investigate the differences of active components in LD vs LB and LD vs LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCA), and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis.

Results

The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD vs LB group and 298 SDMs in LD vs LL group. Compared with LD group, the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCA of transcriptome indicated that there was significant difference in LB vs LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD vs LB group and 18 958 DEGs in LD vs LL group were identified. Notably, CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected, and comparing with LD, CYP724, CYP710A, and CYP734A1 in LB and CYP90B in LL were all up-regulated.

Conclusion

This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of Lilii Bulbus, which can provide new insights for Lilii Bulbus used in the research and development of classic famous formula.

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代谢组和转录组的整合分析为了解莉莉牛的功能成分提供了新的视角
目的 棕百合(Lilium brownii var.viridulum,LB)和白百合(Lilium brownii var.viridulum,LL)是中国药用百合的主要来源。然而,这两种百合中具有治疗功效的功能成分尚不明确。方法通过代谢组和转录组研究LD与LB、LD与LL中有效成分的差异。采用主成分分析(PCA)、层次聚类分析(HCA)等多种分析方法对代谢组和转录组数据进行分析。结果 代谢组的 PCA 和 HCA 分析表明,与 LD 相比,LL 和 LB 中的代谢物明显分离且差异很大。在 LD 组和 LB 组中,有 318 个代谢物存在明显差异(SDMs),在 LD 组和 LL 组中,有 298 个代谢物存在明显差异(SDMs)。与 LD 组相比,LB 组和 LL 组的甾体皂甙和甾体生物碱都有明显的上调,尤其是在 LB 组。转录组的 HCA 显示,LB 组与 LD 组之间存在显著差异,而 LL 组与 LD 组之间的差异略有不同。此外,在 LD 组与 LB 组中发现了 47 540 个 DEGs,在 LD 组与 LL 组中发现了 18 958 个 DEGs。与 LD 组相比,LB 组的 CYP724、CYP710A 和 CYP734A1 以及 LL 组的 CYP90B 均上调。
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来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
期刊介绍: Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included. CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.
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