Priscila R. Nunes , Pedro F. Oliveira , Irene Rebelo , Valeria C. Sandrim , Marco G. Alves
{"title":"Relevance of real-time analyzers to determine mitochondrial quality in endothelial cells and oxidative stress in preeclampsia","authors":"Priscila R. Nunes , Pedro F. Oliveira , Irene Rebelo , Valeria C. Sandrim , Marco G. Alves","doi":"10.1016/j.vph.2024.107372","DOIUrl":null,"url":null,"abstract":"<div><p>Oxidative stress and mitochondrial dysfunction are important elements for the pathophysiology of preeclampsia (PE), a multisystemic hypertensive syndrome of pregnancy, characterized by endothelial dysfunction and responsible for a large part of maternal and fetal morbidity and mortality worldwide. Researchers have dedicated their efforts to unraveling the intricate ways in which certain molecules influence both energy metabolism and oxidative stress. <strong>Exploring</strong> established methodologies from existing literature, <strong>shows that</strong> these investigations predominantly focus on the placenta, identified as a pivotal source that <strong>drives</strong> the changes observed in the disease. In this review, we discuss the role of oxidative stress in pathophysiology of PE, as well as metabolic/endothelial dysfunction. We further discuss the use of seahorse analyzers to study real-time bioenergetics of endothelial cells. Although the benefits are clear, few studies have presented results using this method to assess mitochondrial metabolism in these cells. We performed a search on MEDLINE/PubMed using the terms “Seahorse assay and endothelial dysfunction in HUVEC” as well as “Seahorse assay and preeclampsia”. From our research, we selected 16 original peer-review papers for discussion. Notably, the first search retrieved studies involving <strong>Human Umbilical Vein Endothelial Cells</strong> (HUVECs) but none investigating bioenergetics in PE while the second search retrieved studies exploring the technique in PE but none of the studies used HUVECs. Additional studies are required to investigate real-time mitochondrial bioenergetics in PE. Clearly, there is a need for more complete studies to examine the nuances of mitochondrial bioenergetics, focusing on the contributions of HUVECs in the context of PE.</p></div>","PeriodicalId":23949,"journal":{"name":"Vascular pharmacology","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1537189124000971/pdfft?md5=070d4d7d762c8f7bd7b701296ed602b8&pid=1-s2.0-S1537189124000971-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vascular pharmacology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1537189124000971","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
Oxidative stress and mitochondrial dysfunction are important elements for the pathophysiology of preeclampsia (PE), a multisystemic hypertensive syndrome of pregnancy, characterized by endothelial dysfunction and responsible for a large part of maternal and fetal morbidity and mortality worldwide. Researchers have dedicated their efforts to unraveling the intricate ways in which certain molecules influence both energy metabolism and oxidative stress. Exploring established methodologies from existing literature, shows that these investigations predominantly focus on the placenta, identified as a pivotal source that drives the changes observed in the disease. In this review, we discuss the role of oxidative stress in pathophysiology of PE, as well as metabolic/endothelial dysfunction. We further discuss the use of seahorse analyzers to study real-time bioenergetics of endothelial cells. Although the benefits are clear, few studies have presented results using this method to assess mitochondrial metabolism in these cells. We performed a search on MEDLINE/PubMed using the terms “Seahorse assay and endothelial dysfunction in HUVEC” as well as “Seahorse assay and preeclampsia”. From our research, we selected 16 original peer-review papers for discussion. Notably, the first search retrieved studies involving Human Umbilical Vein Endothelial Cells (HUVECs) but none investigating bioenergetics in PE while the second search retrieved studies exploring the technique in PE but none of the studies used HUVECs. Additional studies are required to investigate real-time mitochondrial bioenergetics in PE. Clearly, there is a need for more complete studies to examine the nuances of mitochondrial bioenergetics, focusing on the contributions of HUVECs in the context of PE.
氧化应激和线粒体功能障碍是子痫前期(PE)病理生理学的重要因素,子痫前期是一种多系统妊娠高血压综合征,以血管内皮功能障碍为特征,是全球大部分孕产妇和胎儿发病和死亡的原因。研究人员致力于揭示某些分子影响能量代谢和氧化应激的复杂方式。通过对现有文献中的既定方法进行探讨,我们发现这些研究主要集中在胎盘上,而胎盘被认为是驱动疾病发生变化的关键源头。在这篇综述中,我们讨论了氧化应激在 PE 病理生理学中的作用,以及代谢/内皮功能障碍。我们进一步讨论了使用海马分析仪研究内皮细胞的实时生物能。虽然这种方法的优点显而易见,但很少有研究利用这种方法来评估这些细胞的线粒体代谢。我们使用 "海马测定和 HUVEC 内皮功能障碍 "以及 "海马测定和子痫前期 "这两个词在 MEDLINE/PubMed 上进行了搜索。通过研究,我们选择了 16 篇同行评审的原创论文进行讨论。值得注意的是,第一次搜索检索到的研究涉及人脐静脉内皮细胞(HUVECs),但没有研究 PE 中的生物能;第二次搜索检索到的研究探讨了 PE 中的生物能技术,但没有研究使用 HUVECs。要研究 PE 中线粒体的实时生物能,还需要进行更多的研究。显然,有必要进行更全面的研究,以检查线粒体生物能的细微差别,重点关注 HUVECs 在 PE 中的贡献。
期刊介绍:
Vascular Pharmacology publishes papers, which contains results of all aspects of biology and pharmacology of the vascular system.
Papers are encouraged in basic, translational and clinical aspects of Vascular Biology and Pharmacology, utilizing approaches ranging from molecular biology to integrative physiology. All papers are in English.
The Journal publishes review articles which include vascular aspects of thrombosis, inflammation, cell signalling, atherosclerosis, and lipid metabolism.