Eduardo Santos, Jonatha Demetrio Gozetto, Eduardo de Almeida, Marcos Augusto Stolf Brasil, Nicolas Gustavo da Cruz da Silva, Vinicius Pires Rezende, Higor José Freitas Alves da Silva, Julia Rosatto Brandão, Gabriel Sgarbiero Montanha, José Lavres and Hudson Wallace Pereira de Carvalho*,
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引用次数: 0
Abstract
X-ray fluorescence spectroscopy (XRF) is an analytical technique employed to determine the elemental composition of diverse materials. Due to its nondestructive nature and direct analysis that requires little or no sample preparation, it has been particularly useful for investigating the mineral composition of plants and soil. However, commercially available XRF benchtop equipment often restricts this type of experiment in plant science due to the volume of the sample chamber and the source–detector geometry. To overcome this problem, we developed an XRF setup that prioritizes in vivo-based experiments. The equipment is equipped with a 4 W Ag X-ray tube and a silicon drift detector. The detection limits are comparable to those of commercial instruments and suitable for evaluating plant tissues. Finally, a case study using tomato plants as a model species and rubidium (Rb+) and strontium (Sr2+) as tracers for potassium (K+) and calcium (Ca2+), respectively, demonstrated their feasibility for long-term in vivo analysis. Therefore, the present XRF system stands out as a viable and cost-effective tool for assessing the absorption and transport of minerals in plant tissues probed by time-resolved in vivo X-ray spectroscopy.
X 射线荧光光谱法(XRF)是一种用于测定各种材料元素组成的分析技术。由于 X 射线荧光光谱具有无损性质,而且直接分析几乎不需要样品制备,因此在研究植物和土壤的矿物成分方面特别有用。然而,由于样品室的体积和源-探测器的几何形状,市面上的 XRF 台式设备往往限制了植物科学中的这类实验。为了克服这一问题,我们开发了一种 XRF 设备,优先考虑基于活体的实验。该设备配备了一个 4 W 银 X 射线管和一个硅漂移探测器。其检测限与商用仪器相当,适合评估植物组织。最后,以番茄植物为模型物种,用铷(Rb+)和锶(Sr2+)分别作为钾(K+)和钙(Ca2+)的示踪剂进行的案例研究证明了它们在长期体内分析中的可行性。因此,本 XRF 系统是通过时间分辨活体 X 射线光谱法评估植物组织中矿物质的吸收和迁移的一种可行且具有成本效益的工具。