Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections

IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Synthetic and Systems Biotechnology Pub Date : 2024-04-10 DOI:10.1016/j.synbio.2024.04.002
Tiffany Chiu , Yanran Li
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Abstract

Polygalacturonase inhibiting proteins (PGIPs) are plant proteins involved in the inhibition of polygalacturonases (PGs), cell-wall degrading enzymes often secreted by phytopathogenic fungi. Previously, we confirmed that PGIP2 from Phaseolus vulgaris (PvPGIP2) can inhibit the growth of Aspergillus niger and Botrytis cinerea on agar plate. In this study, we further validated the feasibility of using PGIP as an environmental and ecological friendly agent to prevent fungal infection post-harvest. We found that application of either purified PGIP (full length PvPGIP2 or truncated tPvPGIP2_5–8), or PGIP-secreting Saccharomyces cerevisiae strains can effectively inhibit fungal growth and necrotic lesions on tobacco leaf. We also examined the effective amount and thermostability of PGIP when applied on plants. A concentration of 0.75 mg/mL or higher can significantly reduce the area of B. cinerea lesions. The activity of full-length PvPGIPs is not affected after incubation at various temperatures ranging from −20 to 42 °C for 24 h, while truncated tPvPGIP2_5–8 lost some efficacy after incubation at 42 °C. Furthermore, we have also examined the efficacy of PGIP on tomato fruit. When the purified PvPGIP2 proteins were applied to tomato fruit inoculated with B. cinerea at a concentration of roughly 1.0 mg/mL, disease incidence and area of disease had reduced by more than half compared to the controls without PGIP treatment. This study explores the potential of PGIPs as exogenously applied, eco-friendly fungal control agents on fruit and vegetables post-harvest.

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聚半乳糖醛酸酶抑制蛋白作为外源应用的天然解决方案,可预防收获后真菌感染
多聚半乳糖醛酸酶抑制蛋白(PGIPs)是一种参与抑制多聚半乳糖醛酸酶(PGs)的植物蛋白,PGs是植物病原真菌经常分泌的细胞壁降解酶。此前,我们证实了来自低等植物的 PGIP2(PvPGIP2)能抑制黑曲霉和灰霉病菌在琼脂平板上的生长。在本研究中,我们进一步验证了使用 PGIP 作为环境和生态友好剂来防止收获后真菌感染的可行性。我们发现,应用纯化的 PGIP(全长 PvPGIP2 或截短的 tPvPGIP2_5-8)或分泌 PGIP 的酿酒酵母菌株能有效抑制烟草叶片上真菌的生长和坏死病变。我们还研究了 PGIP 施用在植物上的有效量和热稳定性。0.75 毫克/毫升或更高浓度的 PGIP 能显著减少烟草真菌病害的面积。全长 PvPGIP 在 -20 至 42 °C 的不同温度下孵育 24 小时后,其活性不受影响,而截短的 tPvPGIP2_5-8 在 42 °C 温度下孵育后会失去一些功效。此外,我们还研究了 PGIP 对番茄果实的功效。当纯化的 PvPGIP2 蛋白以大约 1.0 毫克/毫升的浓度施用到接种了 B. cinerea 的番茄果实上时,与未施用 PGIP 的对照组相比,病害发生率和病害面积减少了一半以上。这项研究探讨了 PGIP 作为外源应用的生态友好型真菌控制剂在水果和蔬菜采收后的应用潜力。
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来源期刊
Synthetic and Systems Biotechnology
Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
6.90
自引率
12.50%
发文量
90
审稿时长
67 days
期刊介绍: Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.
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