Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics

IF 3 4区医学 Q3 ENGINEERING, BIOMEDICAL Biomedical Microdevices Pub Date : 2024-04-09 DOI:10.1007/s10544-024-00703-4

Masahiro Futami, Hiroki Naito, Satoshi Ninomiya, Lee Chuin Chen, Tomohiko Iwano, Kentaro Yoshimura, Yoshiaki Ukita

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Abstract

We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device effectively removed the protein aggregates from the sample, and the performance was comparable to those of conventional manual sample preparation procedures that use high-speed centrifugation. In addition, probe electrospray ionization mass spectrometry (PESI-MS) was performed to compare the device-treated and manually treated samples. The obtained PESI-MS spectra were analyzed by partial least squares discriminant analysis, and the preparation capability of the device was found to be equivalent to that of the conventional method.

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基于 CLOCK 控制的自主离心微流控技术的电喷雾离子化质谱自动样品制备技术

我们报告了一种离心微流控装置，它能在稳态旋转状态下自动进行样品制备，用于质谱分析的临床应用。该自主微流控装置是为控制离心水动力学（CLOCK）范式的液体操作而设计的。所报告的设备高度稳定，在制备过程中，每个单元操作（样品提取、混合和上清液提取）的时间变化小于 7%。该装置采用了带气泡的搅拌机制来混合样品和有机溶剂。我们证实，该装置能有效去除样品中的蛋白质聚集体，其性能与使用高速离心的传统手动样品制备程序相当。此外，我们还进行了探针电喷雾离子化质谱（PESI-MS）分析，以比较装置处理过的样品和人工处理过的样品。通过偏最小二乘法判别分析获得的 PESI-MS 图谱，发现该装置的制备能力与传统方法相当。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.